A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels; Richard S. Taylor; Ross Dobie; Sarah Salisbury; James J. Furniss; Emily Clark; Daniel J. Macqueen; Diego Robledo

doi:10.1371/journal.pone.0285020

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A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: rose.daniels@roslin.ed.ac.uk

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom

https://orcid.org/0000-0002-6702-5304

⨯
Richard S. Taylor,

Roles Formal analysis, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom
⨯
Ross Dobie,

Roles Conceptualization, Investigation

Affiliation Centre for Inflammation Research, The Queen’s Medical Research Institute, Edinburgh BioQuarter, University of Edinburgh, Edinburgh, United Kingdom
⨯
Sarah Salisbury,

Roles Methodology

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom
⨯
James J. Furniss,

Roles Methodology

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom

https://orcid.org/0000-0001-5643-2070

⨯
Emily Clark,

Roles Methodology

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom
⨯
Daniel J. Macqueen,

Roles Conceptualization, Investigation, Methodology, Supervision, Validation, Writing – original draft, Writing – review & editing

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom
⨯
Diego Robledo

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Edinburgh, United Kingdom
⨯

A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels,
Richard S. Taylor,
Ross Dobie,
Sarah Salisbury,
James J. Furniss,
Emily Clark,
Daniel J. Macqueen,
Diego Robledo

Published: September 7, 2023
https://doi.org/10.1371/journal.pone.0285020

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