Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel

Ha T. V. Bui; Huyen T. Bui; Son V. Chu; Huyen T. Nguyen; Anh T. V. Nguyen; Phuong T. Truong; Thang T. H. Dang; Anh T. V. Nguyen

doi:10.1371/journal.pone.0282439

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Open Access

Peer-reviewed

Research Article

Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel

Ha T. V. Bui,

Roles Conceptualization, Funding acquisition, Methodology, Project administration, Supervision, Writing – review & editing

Affiliation Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, Vietnam
⨯
Huyen T. Bui ,

Roles Data curation, Formal analysis, Investigation, Validation, Writing – original draft

* E-mail: vananhbiolab@gmail.com (ATVN); huyenbuibio@gmail.com (HTB)

Affiliations Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, Vietnam, ANABIO R&D Ltd, Hanoi, Vietnam
⨯
Son V. Chu,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Validation, Visualization, Writing – original draft

Affiliation Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, Vietnam
⨯
Huyen T. Nguyen,

Roles Formal analysis, Investigation

Affiliation ANABIO R&D Ltd, Hanoi, Vietnam
⨯
Anh T. V. Nguyen,

Roles Formal analysis, Investigation

Affiliation Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, Vietnam

https://orcid.org/0000-0003-1215-6910

⨯
Phuong T. Truong,

Roles Investigation, Resources, Validation

Affiliation Department of Microbiology, Bach Mai Hospital, Hanoi, Vietnam
⨯
Thang T. H. Dang,

Roles Investigation, Resources, Validation

Affiliation Department of Microbiology, Hanoi Obstetrics & Gynecology Hospital, Hanoi, Vietnam
⨯
Anh T. V. Nguyen

Roles Conceptualization, Data curation, Formal analysis, Methodology, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: vananhbiolab@gmail.com (ATVN); huyenbuibio@gmail.com (HTB)

Affiliation Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, Vietnam
⨯

Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel

Ha T. V. Bui,
Huyen T. Bui,
Son V. Chu,
Huyen T. Nguyen,
Anh T. V. Nguyen,
Phuong T. Truong,
Thang T. H. Dang,
Anh T. V. Nguyen

Published: March 6, 2023
https://doi.org/10.1371/journal.pone.0282439

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Polymerase chain reaction
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Trichomonas vaginalis
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Neisseria gonorrhoeae
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Signal amplification
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Pathogens
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Sexually transmitted diseases
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Cross reactivity
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Gynecologic infections
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