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Open Access
Peer-reviewed
Lab Protocol
Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells
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Raven M. Osborn,
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliations Translational Biomedical Sciences Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Clinical and Translational Sciences Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Justin Leach,
Roles Conceptualization
Affiliation Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Michelle Zanche,
Roles Data curation
Affiliation Genomics Research Center, Center for Advanced Research Technologies, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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John M. Ashton,
Roles Conceptualization, Methodology, Supervision
Affiliation Genomics Research Center, Center for Advanced Research Technologies, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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ChinYi Chu,
Roles Supervision
Affiliation Department of Pediatrics and Center for Children’s Health Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Juilee Thakar,
Roles Funding acquisition, Methodology, Resources, Supervision, Writing – review & editing
Affiliations Translational Biomedical Sciences Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Clinical and Translational Sciences Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Biophysics, Structural, and Computational Biology Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Stephen Dewhurst,
Roles Funding acquisition, Methodology, Resources, Supervision, Writing – review & editing
Affiliations Clinical and Translational Sciences Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Sonia Rosenberger,
Roles Conceptualization, Supervision, Writing – review & editing
Affiliations Department of Environmental Health and Safety, University of Rochester, Rochester, New York, United States of America, Biosafety Level 3 Facility, Center for Advanced Research Technologies, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Martin Pavelka,
Roles Conceptualization, Investigation, Resources, Supervision, Writing – review & editing
Affiliations Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Biosafety Level 3 Facility, Center for Advanced Research Technologies, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Gloria S. Pryhuber,
Roles Resources
Affiliations Department of Pediatrics and Center for Children’s Health Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Thomas J. Mariani,
Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Writing – review & editing
Affiliation Department of Pediatrics and Center for Children’s Health Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Christopher S. Anderson
Roles Conceptualization, Investigation, Methodology, Supervision, Writing – review & editing
* E-mail: Christopher_Anderson@URMC.Rochester.edu
Affiliations Department of Pediatrics and Center for Children’s Health Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America, Division of Neonatology, Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
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Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells
- Raven M. Osborn,
- Justin Leach,
- Michelle Zanche,
- John M. Ashton,
- ChinYi Chu,
- Juilee Thakar,
- Stephen Dewhurst,
- Sonia Rosenberger,
- Martin Pavelka,
- Gloria S. Pryhuber
- Published: February 24, 2023
- https://doi.org/10.1371/journal.pone.0281898