Technique of flat-mount immunostaining for mapping the olfactory epithelium and counting the olfactory sensory neurons

• Marie Gavid ,

  Contributed equally to this work with: Marie Gavid, Louise Coulomb

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  Affiliations Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France, Department of Otorhinolaryngology, CHU of Saint-Etienne, Saint-Etienne, France

  ⨯
• Louise Coulomb ,

  Contributed equally to this work with: Marie Gavid, Louise Coulomb

  Roles Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Justin Thomas,

  Roles Formal analysis, Methodology, Software, Writing – original draft

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Inès Aouimeur,

  Roles Data curation, Formal analysis, Writing – original draft

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Paul Verhoeven,

  Roles Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation CIRI, GIMAP Team, INSERM U1111, CNRS UMR5308, University of Lyon, University of Saint-Etienne, Saint-Etienne, France

  ⨯
• Marielle Mentek,

  Roles Methodology, Validation, Writing – review & editing

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Jean-Marc Dumollard,

  Roles Conceptualization, Data curation, Methodology, Supervision, Validation

  Affiliations Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France, Department of Pathology, CHU of Saint-Etienne, Saint-Etienne, France

  ⨯
• Fabien Forest,

  Roles Conceptualization, Data curation, Methodology, Supervision, Validation, Writing – review & editing

  Affiliations Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France, Department of Pathology, CHU of Saint-Etienne, Saint-Etienne, France

  https://orcid.org/0000-0001-7582-3923

  ⨯
• Jean-Michel Prades,

  Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Validation, Writing – review & editing

  Affiliation Department of Otorhinolaryngology, CHU of Saint-Etienne, Saint-Etienne, France

  ⨯
• Gilles Thuret,

  Roles Conceptualization, Data curation, Funding acquisition, Methodology, Project administration, Supervision, Validation, Writing – review & editing

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Philippe Gain,

  Roles Funding acquisition, Methodology, Project administration, Supervision, Validation, Writing – review & editing

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  ⨯
• Zhiguo He

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: zhiguo.he@univ-st-etienne.fr

  Affiliation Laboratory BIIO (EA2521), Jean Monnet University, Saint-Etienne, France

  https://orcid.org/0000-0001-5153-617X

  ⨯