Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples

• Katherine A. Maki,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Translational Biobehavioral and Health Disparities Branch, Clinical Center, National Institutes of Health, Bethesda, MD, United States of America

  https://orcid.org/0000-0003-4578-960X

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• Brian Wolff,

  Roles Formal analysis, Methodology, Writing – review & editing

  Affiliation National Institute of Nursing Research, National Institutes of Health, Bethesda, MD, United States of America

  https://orcid.org/0000-0002-0886-823X

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• Leonardo Varuzza,

  Roles Software, Writing – review & editing

  Affiliation Thermo Fisher Scientific, Waltham, MA, United States of America

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• Stefan J. Green,

  Roles Supervision, Writing – review & editing

  Affiliation Genomics and Microbiome Core Facility, Rush University, Chicago, IL, United States of America

  https://orcid.org/0000-0003-2781-359X

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• Jennifer J. Barb

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Resources, Supervision, Writing – review & editing

  * E-mail: barbj@nih.gov

  Affiliation Translational Biobehavioral and Health Disparities Branch, Clinical Center, National Institutes of Health, Bethesda, MD, United States of America

  https://orcid.org/0000-0003-3022-0154

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