Directed differentiation of hPSCs through a simplified lateral plate mesoderm protocol for generation of articular cartilage progenitors

Christopher A. Smith; Paul A. Humphreys; Mark A. Naven; Steven Woods; Fabrizio E. Mancini; Julieta O’Flaherty; Qing-Jun Meng; Susan J. Kimber

doi:10.1371/journal.pone.0280024

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Open Access

Peer-reviewed

Research Article

Directed differentiation of hPSCs through a simplified lateral plate mesoderm protocol for generation of articular cartilage progenitors

Christopher A. Smith ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: sue.kimber@manchester.ac.uk (SJK); christopher.smith-2@manchester.ac.uk (CAS)

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Paul A. Humphreys,

Roles Data curation, Investigation, Methodology

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom

https://orcid.org/0000-0002-1833-4864

⨯
Mark A. Naven,

Roles Data curation, Investigation

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Steven Woods,

Roles Data curation

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Fabrizio E. Mancini,

Roles Data curation, Investigation

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Julieta O’Flaherty,

Roles Investigation, Methodology

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Qing-Jun Meng,

Roles Funding acquisition, Resources, Supervision, Writing – review & editing

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯
Susan J. Kimber

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – review & editing

* E-mail: sue.kimber@manchester.ac.uk (SJK); christopher.smith-2@manchester.ac.uk (CAS)

Affiliation Faculty of Biology, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Medicine and Health, University of Manchester, Manchester, United Kingdom
⨯

Directed differentiation of hPSCs through a simplified lateral plate mesoderm protocol for generation of articular cartilage progenitors

Christopher A. Smith,
Paul A. Humphreys,
Mark A. Naven,
Steven Woods,
Fabrizio E. Mancini,
Julieta O’Flaherty,
Qing-Jun Meng,
Susan J. Kimber

Published: January 27, 2023
https://doi.org/10.1371/journal.pone.0280024

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Cell differentiation
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Mesoderm
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Gene expression
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Collagens
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Articular cartilage
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Chondrocytes
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Cartilage
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Pluripotency
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