Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien; Karine Pradeau-Aubreton; Julien Batisse; Joëlle Mezher; Emma Schenckbecher; Justine Marguin; Eric Ennifar; Marc Ruff

doi:10.1371/journal.pone.0279038

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Open Access

Peer-reviewed

Research Article

Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien ,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: drillien@igbmc.fr (RD); ruff@igbmc.fr (MR)

Affiliation Department of Integrative Structural Biology, IGBMC, University of Strasbourg, CNRS UMR 7104, INSERM U964, Illkirch, France
⨯
Karine Pradeau-Aubreton,

Roles Conceptualization, Formal analysis, Investigation, Methodology

Affiliation Department of Integrative Structural Biology, IGBMC, University of Strasbourg, CNRS UMR 7104, INSERM U964, Illkirch, France
⨯
Julien Batisse,

Roles Investigation, Methodology

Affiliation Department of Integrative Structural Biology, IGBMC, University of Strasbourg, CNRS UMR 7104, INSERM U964, Illkirch, France

https://orcid.org/0000-0003-1285-1083

⨯
Joëlle Mezher,

Roles Formal analysis, Investigation, Methodology

Affiliation Structure et Dynamique des Machines Biomoléculaires, Institut de Biologie Moléculaire et Cellulaire, UPR 9002 CNRS/Université de Strasbourg, Strasbourg, France
⨯
Emma Schenckbecher,

Roles Formal analysis, Investigation, Methodology

Affiliation Structure et Dynamique des Machines Biomoléculaires, Institut de Biologie Moléculaire et Cellulaire, UPR 9002 CNRS/Université de Strasbourg, Strasbourg, France
⨯
Justine Marguin,

Roles Formal analysis, Investigation

Affiliation Structure et Dynamique des Machines Biomoléculaires, Institut de Biologie Moléculaire et Cellulaire, UPR 9002 CNRS/Université de Strasbourg, Strasbourg, France
⨯
Eric Ennifar,

Roles Conceptualization, Funding acquisition, Supervision

Affiliation Structure et Dynamique des Machines Biomoléculaires, Institut de Biologie Moléculaire et Cellulaire, UPR 9002 CNRS/Université de Strasbourg, Strasbourg, France
⨯
Marc Ruff

Roles Conceptualization, Funding acquisition, Supervision, Validation, Writing – review & editing

* E-mail: drillien@igbmc.fr (RD); ruff@igbmc.fr (MR)

Affiliation Department of Integrative Structural Biology, IGBMC, University of Strasbourg, CNRS UMR 7104, INSERM U964, Illkirch, France
⨯

Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien,
Karine Pradeau-Aubreton,
Julien Batisse,
Joëlle Mezher,
Emma Schenckbecher,
Justine Marguin,
Eric Ennifar,
Marc Ruff

Published: December 15, 2022
https://doi.org/10.1371/journal.pone.0279038

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Cell cultures
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Protein complexes
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HIV-1
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Plasmid construction
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Protein expression
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Recombinant proteins
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Yellow fluorescent protein
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Recombinant protein purification
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