Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail

• Dai Hatakeyama ,

  Contributed equally to this work with: Dai Hatakeyama, Nozomi Chikamoto

  Roles Conceptualization, Data curation, Methodology, Supervision, Validation, Writing – original draft

  * E-mail: daihatake926@ph.bunri-u.ac.jp (DH); eito@waseda.jp (EI)

  Affiliation Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima City, Japan

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• Nozomi Chikamoto ,

  Contributed equally to this work with: Dai Hatakeyama, Nozomi Chikamoto

  Roles Data curation, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Department of Biology, Waseda University, Tokyo, Japan

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• Kanta Fujimoto,

  Roles Data curation, Investigation, Methodology, Visualization, Writing – review & editing

  Affiliation Department of Biology, Waseda University, Tokyo, Japan

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• Takashi Kitahashi,

  Roles Data curation, Methodology, Validation, Writing – review & editing

  Affiliation Kushiro Nature Conservation Office, Ministry of the Environment Government of Japan, Kushiro City, Japan

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• Etsuro Ito

  Roles Conceptualization, Data curation, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: daihatake926@ph.bunri-u.ac.jp (DH); eito@waseda.jp (EI)

  Affiliations Department of Biology, Waseda University, Tokyo, Japan, Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan

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