Evaluation of culture conditions for osteoclastogenesis in RAW264.7 cells

Yin Cheng; Haixia Liu; Jing Li; Yujie Ma; Changheng Song; Yuhan Wang; Pei Li; Yanjing Chen; Zhiguo Zhang

doi:10.1371/journal.pone.0277871

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Open Access

Peer-reviewed

Research Article

Evaluation of culture conditions for osteoclastogenesis in RAW264.7 cells

Yin Cheng ,

Contributed equally to this work with: Yin Cheng, Haixia Liu

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Haixia Liu ,

Contributed equally to this work with: Yin Cheng, Haixia Liu

Roles Formal analysis, Writing – original draft

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China

https://orcid.org/0000-0003-2198-1015

⨯
Jing Li,

Roles Investigation, Methodology

Affiliation Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences, Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy (BZ0381), Beijing, China
⨯
Yujie Ma,

Roles Data curation, Investigation

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Changheng Song,

Roles Formal analysis, Visualization

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Yuhan Wang,

Roles Data curation, Validation

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Pei Li,

Roles Validation

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Yanjing Chen ,

Roles Supervision, Visualization

* E-mail: zzgtcm@163.com (ZZ); chenyj010@163.com (YC)

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯
Zhiguo Zhang

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

* E-mail: zzgtcm@163.com (ZZ); chenyj010@163.com (YC)

Affiliation Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China
⨯

Evaluation of culture conditions for osteoclastogenesis in RAW264.7 cells

Yin Cheng,
Haixia Liu,
Jing Li,
Yujie Ma,
Changheng Song,
Yuhan Wang,
Pei Li,
Yanjing Chen,
Zhiguo Zhang

Published: November 17, 2022
https://doi.org/10.1371/journal.pone.0277871

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Osteoclasts
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Bone resorption
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Cell differentiation
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Gene expression
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Cell staining
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Bone remodeling
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Macrophages
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Recombinant proteins
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