Experimental factors that impact Ca_V1.2 channel pharmacology—Effects of recording temperature, charge carrier, and quantification of drug effects on the step and ramp currents elicited by the “step-step-ramp” voltage protocol

• Ming Ren ,

  Contributed equally to this work with: Ming Ren, Aaron L. Randolph

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America

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• Aaron L. Randolph ,

  Contributed equally to this work with: Ming Ren, Aaron L. Randolph

  Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliations Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America, Nanion Technologies Inc, Livingston, New Jersey, United States of America

  https://orcid.org/0000-0003-1222-6181

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• Claudia Alvarez-Baron,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America

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• Donglin Guo,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliation Division of Cardiology and Nephrology, Office of Cardiology, Hematology, Endocrinology and Nephrology, Office of New Drugs, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America

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• Phu N. Tran,

  Roles Data curation, Investigation, Writing – review & editing

  Affiliations Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America, Division of Immunology and Hematology Devices, Center for Devices and Radiological Health, US Food and Drug Administration. Silver Spring, Maryland, United States of America

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• Nicolas Thiebaud,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliations Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America, Vertex Pharmaceuticals (Europe) Ltd, Abingdon, Oxfordshire, United Kingdom

  https://orcid.org/0000-0002-4354-7423

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• Jiansong Sheng,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliations Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America, CiPA Lab, Gaithersburg, Maryland, United States of America

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• Jun Zhao,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliation Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America

  https://orcid.org/0000-0003-3663-1595

  ⨯
• Wendy W. Wu

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: wendy.wu@fda.hhs.gov

  Affiliation Division of Applied Regulatory Science, Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, United States of America

  https://orcid.org/0000-0002-5055-4840

  ⨯