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Open Access
Peer-reviewed
Research Article
Direct RNA sequencing of respiratory syncytial virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundance
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I’ah Donovan-Banfield,
Roles Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliations School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom, Department of Infection Biology and Microbiome, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom
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Rachel Milligan,
Roles Investigation, Writing – review & editing
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Sophie Hall,
Roles Investigation, Writing – review & editing
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Tianyi Gao,
Roles Investigation, Writing – review & editing
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Eleanor Murphy,
Roles Investigation, Writing – review & editing
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Jack Li,
Roles Investigation, Writing – review & editing
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Ghada T. Shawli,
Roles Investigation, Writing – review & editing
Affiliation Department of Infection Biology and Microbiome, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom
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Julian Hiscox,
Roles Conceptualization, Writing – original draft, Writing – review & editing
Affiliation Department of Infection Biology and Microbiome, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom
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Xiaodong Zhuang,
Roles Investigation, Methodology, Writing – review & editing
Affiliation Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
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Jane A. McKeating,
Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing
Affiliations Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom, Chinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford, United Kingdom
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Rachel Fearns ,
Roles Conceptualization, Formal analysis, Writing – original draft, Writing – review & editing
* E-mail: d.a.matthews@bristol.ac.uk (DAM); rfearns@bu.edu (RF)
Affiliation Department of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston, Massachusetts, United States of America
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David A. Matthews
Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: d.a.matthews@bristol.ac.uk (DAM); rfearns@bu.edu (RF)
Affiliation School of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United Kingdom
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Direct RNA sequencing of respiratory syncytial virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundance
- I’ah Donovan-Banfield,
- Rachel Milligan,
- Sophie Hall,
- Tianyi Gao,
- Eleanor Murphy,
- Jack Li,
- Ghada T. Shawli,
- Julian Hiscox,
- Xiaodong Zhuang,
- Jane A. McKeating
- Published: November 10, 2022
- https://doi.org/10.1371/journal.pone.0276697