Optimization and validation of an ELISA assay for the determination of antibody responses to CN54gp140 and AIDSVAX BE for use in the Phase IIb PrEPVacc vaccine trial

Ben Gombe; Claire Streatfield; Lorna Leal; Solomon Opio; Sarah Joseph; Jonathan Weber; Jonathan Hare; Pontiano Kaleebu; Jennifer Serwanga

doi:10.1371/journal.pone.0275927

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Open Access

Peer-reviewed

Research Article

Optimization and validation of an ELISA assay for the determination of antibody responses to CN54gp140 and AIDSVAX BE for use in the Phase IIb PrEPVacc vaccine trial

Ben Gombe,

Roles Formal analysis, Methodology, Validation, Writing – original draft

Affiliations MRC/UVRI and LSHTM Uganda Research Unit, Entebbe, Uganda, Uganda Virus Research Institute, Entebbe, Uganda
⨯
Claire Streatfield,

Roles Conceptualization, Data curation, Formal analysis, Methodology, Writing – review & editing

Affiliation IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom
⨯
Lorna Leal,

Roles Data curation, Methodology, Writing – review & editing

Affiliations IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom, AIDS and HIV Infection Research Group, IDIBAPS, Barcelona, Spain
⨯
Solomon Opio,

Roles Data curation, Methodology

Affiliations MRC/UVRI and LSHTM Uganda Research Unit, Entebbe, Uganda, Uganda Virus Research Institute, Entebbe, Uganda
⨯
Sarah Joseph,

Roles Writing – review & editing

Affiliation IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom
⨯
Jonathan Weber,

Roles Conceptualization, Funding acquisition, Supervision

Affiliation Faculty of Medicine, Imperial College, London, United Kingdom
⨯
Jonathan Hare,

Roles Conceptualization, Methodology, Validation, Writing – original draft

Affiliations IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom, IAVI, New York, New York, United States of America

https://orcid.org/0000-0002-1861-4815

⨯
Pontiano Kaleebu,

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

Affiliations MRC/UVRI and LSHTM Uganda Research Unit, Entebbe, Uganda, Uganda Virus Research Institute, Entebbe, Uganda, London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom
⨯
Jennifer Serwanga

Roles Conceptualization, Formal analysis, Supervision, Writing – review & editing

* E-mail: Jennifer.serwanga@mrcuganda.org

Affiliations MRC/UVRI and LSHTM Uganda Research Unit, Entebbe, Uganda, Uganda Virus Research Institute, Entebbe, Uganda
⨯

Optimization and validation of an ELISA assay for the determination of antibody responses to CN54gp140 and AIDSVAX BE for use in the Phase IIb PrEPVacc vaccine trial

Ben Gombe,
Claire Streatfield,
Lorna Leal,
Solomon Opio,
Sarah Joseph,
Jonathan Weber,
Jonathan Hare,
Pontiano Kaleebu,
Jennifer Serwanga

Published: November 3, 2022
https://doi.org/10.1371/journal.pone.0275927

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