A single application of fertilizer can affect semi-natural grassland vegetation over half a century

• Michio Tsutsumi ,

  Contributed equally to this work with: Michio Tsutsumi, Syuntaro Hiradate

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

  * E-mail: mcot@affrc.go.jp

  Affiliation Western Region Agricultural Research Center (Kinki, Chugoku and Shikoku Regions), National Agriculture and Food Research Organization, Shimane, Japan

  https://orcid.org/0000-0003-0838-0944

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• Syuntaro Hiradate ,

  Contributed equally to this work with: Michio Tsutsumi, Syuntaro Hiradate

  Roles Conceptualization, Data curation, Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Faculty of Agriculture, Kyushu University, Fukuoka, Japan

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• Masashi Yokogawa,

  Roles Conceptualization, Funding acquisition, Investigation, Writing – original draft, Writing – review & editing

  Affiliation Osaka Museum of Natural History, Osaka, Japan

  https://orcid.org/0000-0003-3227-0542

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• Eri Yamakita,

  Roles Data curation, Investigation, Writing – review & editing

  Affiliation Faculty of Agriculture, Kyushu University, Fukuoka, Japan

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• Masahito Inoue,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation The Shimane Nature Museum of Mt. Sanbe, Shimane, Japan

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• Yoshitaka Takahashi

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Japan Grassland Conservation Network, Shimane, Japan

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