A simplified function-first method for the discovery and optimization of bispecific immune engaging antibodies

Alex Shepherd; Bigitha Bennychen; Anne Marcil; Darin Bloemberg; Robert A. Pon; Risini D. Weeratna; Scott McComb

doi:10.1371/journal.pone.0273884

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Open Access

Peer-reviewed

Research Article

A simplified function-first method for the discovery and optimization of bispecific immune engaging antibodies

Alex Shepherd,

Roles Formal analysis, Investigation, Methodology, Writing – original draft

Affiliations Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada
⨯
Bigitha Bennychen,

Roles Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliations Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada
⨯
Anne Marcil,

Roles Conceptualization, Formal analysis, Investigation, Project administration, Resources, Supervision, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada
⨯
Darin Bloemberg,

Roles Conceptualization, Formal analysis, Investigation, Methodology, Project administration, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada
⨯
Robert A. Pon,

Roles Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada
⨯
Risini D. Weeratna,

Roles Formal analysis, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada
⨯
Scott McComb

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

* E-mail: scott.mccomb@nrc-cnrc.gc.ca

Affiliations Human Health Therapeutics Research Centre, National Research Council, Ottawa, Canada, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada, Centre for Infection, Immunity and Inflammation, University of Ottawa, Ottawa, Canada

https://orcid.org/0000-0002-4616-7864

⨯

A simplified function-first method for the discovery and optimization of bispecific immune engaging antibodies

Alex Shepherd,
Bigitha Bennychen,
Anne Marcil,
Darin Bloemberg,
Robert A. Pon,
Risini D. Weeratna,
Scott McComb

Published: June 22, 2023
https://doi.org/10.1371/journal.pone.0273884

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