Development and diagnostic validation of a one-step multiplex RT-PCR assay as a rapid method to detect and identify Nervous Necrosis Virus (NNV) and its variants circulating in the Mediterranean

• Francesca Errani ,

  Contributed equally to this work with: Francesca Errani, Enrico Volpe

  Roles Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Cesenatico (FC), Italy

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• Enrico Volpe ,

  Contributed equally to this work with: Francesca Errani, Enrico Volpe

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Visualization, Writing – review & editing

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Cesenatico (FC), Italy

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• Enrique Riera-Ferrer,

  Roles Investigation, Methodology, Writing – original draft, Writing – review & editing

  Affiliation Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain

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• Monica Caffara,

  Roles Investigation, Writing – review & editing

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano dell’Emilia (BO), Italy

  https://orcid.org/0000-0003-3429-6121

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• Francesc Padrós,

  Roles Funding acquisition, Writing – review & editing

  Affiliation Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain

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• Andrea Gustinelli,

  Roles Investigation, Resources, Writing – review & editing

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano dell’Emilia (BO), Italy

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• Marialetizia Fioravanti,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Ozzano dell’Emilia (BO), Italy

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• Sara Ciulli

  Roles Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Writing – original draft, Writing – review & editing

  * E-mail: sara.ciulli@unibo.it

  Affiliation Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Cesenatico (FC), Italy

  https://orcid.org/0000-0003-3371-2488

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