Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles

Charlotte Pronier; Jérémy Bomo; Juliette Besombes; Valentine Genet; Syria Laperche; Philippe Gripon; Vincent Thibault

doi:10.1371/journal.pone.0272474

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Open Access

Peer-reviewed

Research Article

Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles

Charlotte Pronier,

Roles Formal analysis, Methodology, Supervision, Validation, Writing – original draft, Writing – review & editing

Affiliation Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France
⨯
Jérémy Bomo,

Roles Data curation, Investigation, Validation, Visualization, Writing – review & editing

Affiliation Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France

https://orcid.org/0000-0002-3065-449X

⨯
Juliette Besombes,

Roles Investigation, Validation, Writing – review & editing

Affiliation Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France
⨯
Valentine Genet,

Roles Data curation, Formal analysis, Methodology

Affiliation Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France
⨯
Syria Laperche,

Roles Methodology, Resources

Affiliations Department of Blood-Borne Agents, National Reference Center of Infectious Risks in Blood Transfusion, Institut National de la Transfusion Sanguine, Paris, France, Etablissement Français du Sang, La Plaine-Saint-Denis, France
⨯
Philippe Gripon,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France
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Vincent Thibault

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: vincent.thibault@univ-rennes1.fr

Affiliation Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) UMR_S 1085, Rennes, France

https://orcid.org/0000-0001-6517-2901

⨯

Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles

Charlotte Pronier,
Jérémy Bomo,
Juliette Besombes,
Valentine Genet,
Syria Laperche,
Philippe Gripon,
Vincent Thibault

Published: November 16, 2022
https://doi.org/10.1371/journal.pone.0272474

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Capsids
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Blood plasma
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Virions
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Sucrose
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Phylogeography
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Detergents
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DNA separation
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Hepatitis B virus
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