Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

• Kai Pascal Alexander Hänggeli,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Validation, Writing – original draft

  Affiliations Department of Infectious Diseases and Pathobiology, Institute of Parasitology, University of Bern, Bern, Switzerland, Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland

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• Andrew Hemphill ,

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  * E-mail: ghalia.boubaker@vetsuisse.unibe.ch (GB); andrew.hemphill@vetsuisse.unibe.ch (AH)

  Affiliation Department of Infectious Diseases and Pathobiology, Institute of Parasitology, University of Bern, Bern, Switzerland

  https://orcid.org/0000-0002-0622-2128

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• Norbert Müller,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Infectious Diseases and Pathobiology, Institute of Parasitology, University of Bern, Bern, Switzerland

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• Bernd Schimanski,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Bern, Switzerland

  https://orcid.org/0000-0003-1336-3671

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• Philipp Olias,

  Roles Methodology, Writing – review & editing

  Affiliation Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland

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• Joachim Müller,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Infectious Diseases and Pathobiology, Institute of Parasitology, University of Bern, Bern, Switzerland

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• Ghalia Boubaker

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: ghalia.boubaker@vetsuisse.unibe.ch (GB); andrew.hemphill@vetsuisse.unibe.ch (AH)

  Affiliation Department of Infectious Diseases and Pathobiology, Institute of Parasitology, University of Bern, Bern, Switzerland

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