Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid

• Mariana Brena Souza,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

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• Maria Cecília Cergole-Novella,

  Roles Writing – original draft, Writing – review & editing

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

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• Delma Aparecida Molinari,

  Roles Data curation, Investigation, Methodology

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

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• Daniela Rodrigues Colpas,

  Roles Data curation, Investigation, Methodology

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

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• Andréia Moreira dos Santos Carmo,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

  https://orcid.org/0000-0002-0602-4623

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• Vilma dos Santos Menezes Gaiotto Daros,

  Roles Funding acquisition, Resources, Writing – review & editing

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

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• Ivana Barros de Campos

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: ivanacamp@gmail.com, ivana.campos@ial.sp.gov.br

  Affiliation Santo André Regional Center, Adolfo Lutz Institute, Santo André, São Paulo, Brazil

  https://orcid.org/0000-0003-0334-0572

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