Detection of infiltrating fibroblasts by single-cell transcriptomics in human kidney allografts

Hemant Suryawanshi; Hua Yang; Michelle Lubetzky; Pavel Morozov; Mila Lagman; Gaurav Thareja; Alicia Alonso; Carol Li; Catherine Snopkowski; Aziz Belkadi; Franco B. Mueller; John R. Lee; Darshana M. Dadhania; Steven P. Salvatore; Surya V. Seshan; Vijay K. Sharma; Karsten Suhre; Manikkam Suthanthiran; Thomas Tuschl; Thangamani Muthukumar

doi:10.1371/journal.pone.0267704

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Open Access

Peer-reviewed

Research Article

Detection of infiltrating fibroblasts by single-cell transcriptomics in human kidney allografts

Hemant Suryawanshi ,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: hsuryawans@rockefeller.edu (HS); ttuschl@rockefeller.edu (TT); mut9002@med.cornell.edu (TM)

Affiliation Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, United States of America
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Hua Yang,

Roles Investigation, Methodology

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Michelle Lubetzky,

Roles Investigation

Affiliations Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America, Department of Transplantation Medicine, New York Presbyterian Hospital-Weill Cornell Medical College, New York, NY, United States of America
⨯
Pavel Morozov,

Roles Data curation, Software, Validation, Visualization

Affiliation Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, United States of America
⨯
Mila Lagman,

Roles Conceptualization, Investigation

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Gaurav Thareja,

Roles Formal analysis, Validation, Visualization

Affiliation Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Doha, Qatar

https://orcid.org/0000-0003-2277-6400

⨯
Alicia Alonso,

Roles Data curation, Formal analysis, Investigation, Methodology, Resources

Affiliation Epigenomics Core Facility, Weill Cornell Medical College, New York, NY, United States of America
⨯
Carol Li,

Roles Investigation

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Catherine Snopkowski,

Roles Investigation

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Aziz Belkadi,

Roles Formal analysis, Validation, Visualization

Affiliation Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Doha, Qatar
⨯
Franco B. Mueller,

Roles Investigation

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
John R. Lee,

Roles Investigation

Affiliations Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America, Department of Transplantation Medicine, New York Presbyterian Hospital-Weill Cornell Medical College, New York, NY, United States of America
⨯
Darshana M. Dadhania,

Roles Investigation

Affiliations Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America, Department of Transplantation Medicine, New York Presbyterian Hospital-Weill Cornell Medical College, New York, NY, United States of America
⨯
Steven P. Salvatore,

Roles Formal analysis

Affiliation Division of Renal Pathology, Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Surya V. Seshan,

Roles Formal analysis

Affiliation Division of Renal Pathology, Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Vijay K. Sharma,

Roles Investigation

Affiliation Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America
⨯
Karsten Suhre,

Roles Formal analysis, Resources, Validation, Visualization

Affiliation Department of Physiology and Biophysics, Weill Cornell Medical College in Qatar, Doha, Qatar
⨯
Manikkam Suthanthiran,

Roles Conceptualization, Funding acquisition, Project administration, Resources, Writing – review & editing

Affiliations Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America, Department of Transplantation Medicine, New York Presbyterian Hospital-Weill Cornell Medical College, New York, NY, United States of America
⨯
Thomas Tuschl ,

Roles Conceptualization, Data curation, Funding acquisition, Investigation, Project administration, Resources, Software, Supervision, Writing – original draft, Writing – review & editing

* E-mail: hsuryawans@rockefeller.edu (HS); ttuschl@rockefeller.edu (TT); mut9002@med.cornell.edu (TM)

Affiliation Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, United States of America
⨯
[ ... ],
Thangamani Muthukumar

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Writing – original draft, Writing – review & editing

* E-mail: hsuryawans@rockefeller.edu (HS); ttuschl@rockefeller.edu (TT); mut9002@med.cornell.edu (TM)

Affiliations Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medical College, New York, NY, United States of America, Department of Transplantation Medicine, New York Presbyterian Hospital-Weill Cornell Medical College, New York, NY, United States of America

https://orcid.org/0000-0002-0294-6209

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Detection of infiltrating fibroblasts by single-cell transcriptomics in human kidney allografts

Hemant Suryawanshi,
Hua Yang,
Michelle Lubetzky,
Pavel Morozov,
Mila Lagman,
Gaurav Thareja,
Alicia Alonso,
Carol Li,
Catherine Snopkowski,
Aziz Belkadi

Published: June 3, 2022
https://doi.org/10.1371/journal.pone.0267704

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Kidneys
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Biopsy
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Fibroblasts
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Gene expression
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Renal transplantation
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Transplant rejection
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Fibrosis
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T cells
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