Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditions

• Emerson Glassey,

  Roles Conceptualization, Investigation, Software, Writing – original draft, Writing – review & editing

  Affiliation Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, United States of America

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• Andrew M. King,

  Roles Investigation

  Affiliation Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, United States of America

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• Daniel A. Anderson,

  Roles Software

  Affiliation Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, United States of America

  https://orcid.org/0000-0001-9655-323X

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• Zhengan Zhang,

  Roles Investigation

  Affiliation Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, United States of America

  https://orcid.org/0000-0003-3993-1163

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• Christopher A. Voigt

  Roles Conceptualization, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: cavoigt@gmail.com

  Affiliation Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, United States of America

  https://orcid.org/0000-0003-0844-4776

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