Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish

• Chiho Homma,

  Roles Conceptualization, Data curation, Investigation, Methodology, Validation, Visualization, Writing – original draft

  Affiliation The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan

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• Daiki Inokuchi,

  Roles Conceptualization, Investigation

  Affiliation Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan

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• Yohei Nakamura,

  Roles Conceptualization, Resources, Writing – review & editing

  Affiliation Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan

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• Wilfredo H. Uy,

  Roles Resources

  Affiliation Institute of Fisheries Research and Development, Mindanao State University at Naawan, Naawan, Misamis Oriental, Philippines

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• Kouhei Ohnishi,

  Roles Data curation, Investigation

  Affiliations The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan, Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan

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• Haruo Yamaguchi,

  Roles Data curation

  Affiliations The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan, Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan

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• Masao Adachi

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: madachi@kochi-u.ac.jp

  Affiliations The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan, Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan

  https://orcid.org/0000-0001-6062-3160

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