Comparison of different sequencing techniques for identification of SARS-CoV-2 variants of concern with multiplex real-time PCR

Diyanath Ranasinghe; Tibutius Thanesh Pramanayagam Jayadas; Deshni Jayathilaka; Chandima Jeewandara; Osanda Dissanayake; Dinuka Guruge; Dinuka Ariyaratne; Dumni Gunasinghe; Laksiri Gomes; Ayesha Wijesinghe; Ruwan Wijayamuni; Gathsaurie Neelika Malavige

doi:10.1371/journal.pone.0265220

Loading metrics

Open Access

Peer-reviewed

Research Article

Comparison of different sequencing techniques for identification of SARS-CoV-2 variants of concern with multiplex real-time PCR

Diyanath Ranasinghe ,

Contributed equally to this work with: Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara

Roles Conceptualization, Formal analysis, Investigation, Writing – original draft

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka

https://orcid.org/0000-0001-5151-8908

⨯
Tibutius Thanesh Pramanayagam Jayadas ,

Contributed equally to this work with: Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara

Roles Formal analysis, Investigation, Writing – original draft

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Deshni Jayathilaka ,

Contributed equally to this work with: Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara

Roles Formal analysis, Investigation

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Chandima Jeewandara ,

Contributed equally to this work with: Diyanath Ranasinghe, Tibutius Thanesh Pramanayagam Jayadas, Deshni Jayathilaka, Chandima Jeewandara

Roles Conceptualization, Funding acquisition, Project administration, Resources

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Osanda Dissanayake,

Roles Data curation, Investigation

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka

https://orcid.org/0000-0001-7919-5979

⨯
Dinuka Guruge,

Roles Data curation, Project administration, Resources

Affiliation Colombo Municipal Council, Colombo, Sri Lanka
⨯
Dinuka Ariyaratne,

Roles Investigation, Methodology

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Dumni Gunasinghe,

Roles Investigation, Methodology

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Laksiri Gomes,

Roles Investigation

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Ayesha Wijesinghe,

Roles Investigation

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Ruwan Wijayamuni,

Roles Project administration, Resources

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
⨯
Gathsaurie Neelika Malavige

Roles Conceptualization, Funding acquisition, Writing – original draft, Writing – review & editing

* E-mail: gathsaurie.malavige@ndm.ox.ac.uk

Affiliation Allergy Immunology and Cell Biology Unit, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda, Sri Lanka

https://orcid.org/0000-0001-9201-0449

⨯

Comparison of different sequencing techniques for identification of SARS-CoV-2 variants of concern with multiplex real-time PCR

Diyanath Ranasinghe,
Tibutius Thanesh Pramanayagam Jayadas,
Deshni Jayathilaka,
Chandima Jeewandara,
Osanda Dissanayake,
Dinuka Guruge,
Dinuka Ariyaratne,
Dumni Gunasinghe,
Laksiri Gomes,
Ayesha Wijesinghe

Published: April 4, 2022
https://doi.org/10.1371/journal.pone.0265220

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Single nucleotide polymorphisms
Is the Subject Area "Single nucleotide polymorphisms" applicable to this article?

Thanks for your feedback.
SARS CoV 2
Is the Subject Area "SARS CoV 2" applicable to this article?

Thanks for your feedback.
Genome sequencing
Is the Subject Area "Genome sequencing" applicable to this article?

Thanks for your feedback.
Frameshift mutation
Is the Subject Area "Frameshift mutation" applicable to this article?

Thanks for your feedback.
Genomics
Is the Subject Area "Genomics" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Mutation detection
Is the Subject Area "Mutation detection" applicable to this article?

Thanks for your feedback.
Nucleotide sequencing
Is the Subject Area "Nucleotide sequencing" applicable to this article?

Thanks for your feedback.