High-throughput widefield fluorescence imaging of 3D samples using deep learning for 2D projection image restoration

Edvin Forsgren; Christoffer Edlund; Miniver Oliver; Kalpana Barnes; Rickard Sjögren; Timothy R. Jackson

doi:10.1371/journal.pone.0264241

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Open Access

Peer-reviewed

Research Article

High-throughput widefield fluorescence imaging of 3D samples using deep learning for 2D projection image restoration

Edvin Forsgren ,

Contributed equally to this work with: Edvin Forsgren, Christoffer Edlund

Roles Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: edvin.forsgren@umu.se

Affiliation Computational Life Science Cluster (CLiC), Department of Chemistry, Umeå University, Umeå, Sweden

https://orcid.org/0000-0002-1898-4453

⨯
Christoffer Edlund ,

Contributed equally to this work with: Edvin Forsgren, Christoffer Edlund

Roles Conceptualization, Methodology, Software, Supervision, Writing – original draft, Writing – review & editing

Affiliation Sartorius Corporate Research, Sartorius Stedim Data Analytics AB, Umeå, Sweden
⨯
Miniver Oliver,

Roles Data curation, Resources

Current address: Biopharm Discovery, GlaxoSmithKline, Stevenage, Hertfordshire, United Kingdom

Affiliation Sartorius BioAnalytics, Essen BioScience, Ltd., Units 2 & 3 The Quadrant, Royston, Hertfordshire, United Kingdom
⨯
Kalpana Barnes,

Roles Data curation, Resources

Affiliation Sartorius BioAnalytics, Essen BioScience, Ltd., Units 2 & 3 The Quadrant, Royston, Hertfordshire, United Kingdom
⨯
Rickard Sjögren,

Roles Conceptualization, Supervision, Writing – original draft, Writing – review & editing

Affiliation Sartorius Corporate Research, Sartorius Stedim Data Analytics AB, Umeå, Sweden

https://orcid.org/0000-0001-7881-0968

⨯
Timothy R. Jackson

Roles Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

Affiliation Sartorius BioAnalytics, Essen BioScience, Ltd., Units 2 & 3 The Quadrant, Royston, Hertfordshire, United Kingdom
⨯

High-throughput widefield fluorescence imaging of 3D samples using deep learning for 2D projection image restoration

Edvin Forsgren,
Christoffer Edlund,
Miniver Oliver,
Kalpana Barnes,
Rickard Sjögren,
Timothy R. Jackson

Published: May 19, 2022
https://doi.org/10.1371/journal.pone.0264241

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Fluorescence imaging
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Cancers and neoplasms
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Imaging techniques
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Fluorescence microscopy
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