Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen

• Ator Ashoti ,

  Contributed equally to this work with: Ator Ashoti, Francesco Limone

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  * E-mail: a.r.o.ashoti@uu.nl (AA); francesco_limone@fas.harvard.edu (FL); n.geijsen@lumc.nl (NG); eggan@mcb.harvard.edu (KE)

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Francesco Limone ,

  Contributed equally to this work with: Ator Ashoti, Francesco Limone

  Roles Data curation, Formal analysis

  * E-mail: a.r.o.ashoti@uu.nl (AA); francesco_limone@fas.harvard.edu (FL); n.geijsen@lumc.nl (NG); eggan@mcb.harvard.edu (KE)

  Affiliations Department of Stem Cell and Regenerative Biology, Harvard University Cambridge, MA, United States of America, Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, United States of America

  ⨯
• Melissa van Kranenburg,

  Roles Data curation, Investigation

  Current address: Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Anna Alemany,

  Roles Formal analysis

  Current address: Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Mirna Baak,

  Roles Formal analysis

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Judith Vivié,

  Roles Data curation, Formal analysis

  Affiliations Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands, Single Cell Discoveries, Utrecht, The Netherlands

  ⨯
• Frederica Piccioni,

  Roles Formal analysis

  Affiliation Broad Institute, Cambridge, MA, United States of America

  https://orcid.org/0000-0003-1210-3210

  ⨯
• Pascale F. Dijkers,

  Roles Writing – review & editing

  Current address: Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Menno Creyghton,

  Roles Supervision

  Current address: Department of Developmental Embryology, Erasmus University Medical Centre, Rotterdam, The Netherlands

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  ⨯
• Kevin Eggan ,

  Roles Supervision

  * E-mail: a.r.o.ashoti@uu.nl (AA); francesco_limone@fas.harvard.edu (FL); n.geijsen@lumc.nl (NG); eggan@mcb.harvard.edu (KE)

  Affiliations Department of Stem Cell and Regenerative Biology, Harvard University Cambridge, MA, United States of America, Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, United States of America

  ⨯
• Niels Geijsen

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: a.r.o.ashoti@uu.nl (AA); francesco_limone@fas.harvard.edu (FL); n.geijsen@lumc.nl (NG); eggan@mcb.harvard.edu (KE)

  Current address: Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands

  Affiliation Hubrecht Institute, Developmental Biology and Stem Cell Research, Utrecht, The Netherlands

  https://orcid.org/0000-0003-3592-4353

  ⨯