Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang; Shujuan Zhu; Yu Xing; Qian Liu; Zhen Guo; Ziling Cai; Zihao Shen; Qingqian Xia; Huajun Sheng

doi:10.1371/journal.pone.0263162

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Open Access

Peer-reviewed

Research Article

Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang ,

Contributed equally to this work with: Hao Zhang, Shujuan Zhu

Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Shujuan Zhu ,

Contributed equally to this work with: Hao Zhang, Shujuan Zhu

Roles Software, Supervision, Validation

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Yu Xing,

Roles Conceptualization, Data curation, Funding acquisition, Investigation

Affiliation Department of Forensic Medicine, Chongqing Medical University, Chongqing, China
⨯
Qian Liu,

Roles Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Zhen Guo,

Roles Methodology, Project administration, Resources, Software

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Ziling Cai,

Roles Software, Supervision, Validation, Visualization

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Zihao Shen,

Roles Investigation, Software, Supervision, Validation

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Qingqian Xia,

Roles Conceptualization, Formal analysis, Investigation

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯
Huajun Sheng

Roles Funding acquisition, Investigation, Methodology, Project administration, Visualization, Writing – original draft, Writing – review & editing

* E-mail: 100448@cqmu.edu.cn

Affiliation Department of Human Anatomy, College of Basic Medicine, Chongqing Medical University, Chongqing, China
⨯

Effect of cryopreservation on A172 and U251 glioma cells infected with lentiviral vectors designed for CRISPR/Cas9-mediated aquaporin-8 knock-out

Hao Zhang,
Shujuan Zhu,
Yu Xing,
Qian Liu,
Zhen Guo,
Ziling Cai,
Zihao Shen,
Qingqian Xia,
Huajun Sheng

Published: March 4, 2022
https://doi.org/10.1371/journal.pone.0263162

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Cryopreservation
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Viral transmission and infection
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Glioma cells
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CRISPR
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Lentivirus
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Cell cultures
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Vector-borne diseases
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Viral gene expression
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