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Open Access

Peer-reviewed

Research Article

OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Artis Linars,

Roles Investigation

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia
⨯
Ivars Silamikelis,

Roles Investigation, Software, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia
⨯
Dita Gudra,

Roles Investigation, Resources, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia
⨯
Ance Roga,

Roles Conceptualization, Investigation, Writing – review & editing

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia
⨯
Davids Fridmanis

Roles Conceptualization, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

* E-mail: davids@biomed.lu.lv

Affiliation Latvian Biomedical Research and Study Centre, Riga, Latvia

https://orcid.org/0000-0003-0310-0448

⨯

OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Artis Linars,
Ivars Silamikelis,
Dita Gudra,
Ance Roga,
Davids Fridmanis

x

Published: August 8, 2022
https://doi.org/10.1371/journal.pone.0262968
See the preprint

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Polymerase chain reaction
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Plasmid construction
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Nucleotide sequencing
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Peptide libraries
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Mutagenesis
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Oligonucleotides
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Signal amplification
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Saccharomyces cerevisiae
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