Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure

Stella B. Somiari; Susan Shuss; Jianfang Liu; Kimberly Mamula; Amy O’Donnell; Brenda Deyarmin; Jennifer Kane; Amber Greenawalt; Caroline Larson; Sean Rigby; Hai Hu; Craig D. Shriver

doi:10.1371/journal.pone.0262654

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Open Access

Peer-reviewed

Research Article

Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure

Stella B. Somiari ,

Roles Conceptualization, Methodology, Supervision, Writing – original draft, Writing – review & editing

* E-mail: s.somiari@wriwindber.org

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America

https://orcid.org/0000-0002-7170-9098

⨯
Susan Shuss,

Roles Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Jianfang Liu,

Roles Data curation, Formal analysis, Visualization

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Kimberly Mamula,

Roles Data curation, Formal analysis

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Amy O’Donnell,

Roles Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Brenda Deyarmin,

Roles Data curation, Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Jennifer Kane,

Roles Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Amber Greenawalt,

Roles Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Caroline Larson,

Roles Data curation, Methodology

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Sean Rigby,

Roles Data curation

Affiliation Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America
⨯
Hai Hu,

Roles Formal analysis, Supervision, Writing – review & editing

Affiliations Chan Soon-Shiong Institute of Molecular Medicine at Windber, Windber, Pennsylvania, United States of America, Murtha Cancer Center Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
⨯
Craig D. Shriver

Roles Funding acquisition, Resources

Affiliation Murtha Cancer Center Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
⨯

Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure

Stella B. Somiari,
Susan Shuss,
Jianfang Liu,
Kimberly Mamula,
Amy O’Donnell,
Brenda Deyarmin,
Jennifer Kane,
Amber Greenawalt,
Caroline Larson,
Sean Rigby

Published: January 18, 2022
https://doi.org/10.1371/journal.pone.0262654

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Breast tissue
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RNA isolation
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Tumor resection
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Gene expression
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Nucleic acids
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RNA analysis
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Surgical resection
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Reverse transcriptase-polymerase chain reaction
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