Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling

• Elinor Shvartsman ,

  Contributed equally to this work with: Elinor Shvartsman, Meika E. I. Richmond, John J. Schellenberg

  Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Internal Medicine, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada, JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada

  https://orcid.org/0000-0001-6456-2836

  ⨯
• Meika E. I. Richmond ,

  Contributed equally to this work with: Elinor Shvartsman, Meika E. I. Richmond, John J. Schellenberg

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliations Department of Internal Medicine, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada, JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada

  ⨯
• John J. Schellenberg ,

  Contributed equally to this work with: Elinor Shvartsman, Meika E. I. Richmond, John J. Schellenberg

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliations JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada

  https://orcid.org/0000-0003-3970-1608

  ⨯
• Alana Lamont,

  Roles Data curation, Formal analysis, Investigation, Writing – review & editing

  Affiliations Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, United States of America, Department of Obstetrics and Gynecology, University of Manitoba, Winnipeg, Canada

  ⨯
• Catia Perciani,

  Roles Investigation, Methodology, Project administration

  Affiliation Department of Immunology, University of Toronto, Toronto, Canada

  ⨯
• Justen N. H. Russell,

  Roles Investigation, Methodology

  Affiliations JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Immunology, University of Toronto, Toronto, Canada

  https://orcid.org/0000-0002-5275-0116

  ⨯
• Vanessa Poliquin,

  Roles Investigation, Project administration, Resources, Writing – review & editing

  Affiliation Department of Obstetrics and Gynecology, University of Manitoba, Winnipeg, Canada

  https://orcid.org/0000-0003-0526-3902

  ⨯
• Adam Burgener,

  Roles Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing

  Affiliations Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, United States of America, Department of Obstetrics and Gynecology, University of Manitoba, Winnipeg, Canada, Department of Medicine, Karolinska Institute, Solna, Sweden

  ⨯
• Walter Jaoko,

  Roles Funding acquisition, Project administration, Resources, Supervision

  Affiliation Kenyan AIDS Vaccine Initiative–Institute of Clinical Research (KAVI-ICR), University of Nairobi, Nairobi, Kenya

  ⨯
• Paul Sandstrom,

  Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada

  ⨯
• Kelly S. MacDonald

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: kelly.macdonald@umanitoba.ca

  Affiliations Department of Internal Medicine, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada, JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada, Department of Immunology, University of Toronto, Toronto, Canada

  https://orcid.org/0000-0003-3216-247X

  ⨯