Single gene targeted nanopore sequencing enables simultaneous identification and antimicrobial resistance detection of sexually transmitted infections

Liqing Zhou; Andrea Lopez Rodas; Luz Marina Llangarí; Natalia Romero Sandoval; Philip Cooper; Syed Tariq Sadiq

doi:10.1371/journal.pone.0262242

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Open Access

Peer-reviewed

Research Article

Single gene targeted nanopore sequencing enables simultaneous identification and antimicrobial resistance detection of sexually transmitted infections

Liqing Zhou,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

Affiliation Applied Diagnostic Research and Evaluation Unit, Institute for Infection and Immunity, St George’s, University of London, London, United Kingdom

https://orcid.org/0000-0002-0942-7727

⨯
Andrea Lopez Rodas,

Roles Investigation, Methodology, Validation, Writing – review & editing

Affiliation School of Medicine, Faculty of Health and Life Sciences, Universidad Internacional del Ecuador, Quito, Ecuador
⨯
Luz Marina Llangarí,

Roles Investigation, Methodology, Validation, Writing – review & editing

Affiliation School of Medicine, Faculty of Health and Life Sciences, Universidad Internacional del Ecuador, Quito, Ecuador

https://orcid.org/0000-0002-9387-545X

⨯
Natalia Romero Sandoval,

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Writing – review & editing

Affiliations School of Medicine, Faculty of Health and Life Sciences, Universidad Internacional del Ecuador, Quito, Ecuador, Grups de Recerca d’Amèrica i Àfrica Llatines, GRAAL, Barcelona, Spain
⨯
Philip Cooper,

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Writing – review & editing

Affiliations Applied Diagnostic Research and Evaluation Unit, Institute for Infection and Immunity, St George’s, University of London, London, United Kingdom, School of Medicine, Faculty of Health and Life Sciences, Universidad Internacional del Ecuador, Quito, Ecuador
⨯
Syed Tariq Sadiq

Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

* E-mail: ssadiq@sgul.ac.uk

Affiliation Applied Diagnostic Research and Evaluation Unit, Institute for Infection and Immunity, St George’s, University of London, London, United Kingdom
⨯

Single gene targeted nanopore sequencing enables simultaneous identification and antimicrobial resistance detection of sexually transmitted infections

Liqing Zhou,
Andrea Lopez Rodas,
Luz Marina Llangarí,
Natalia Romero Sandoval,
Philip Cooper,
Syed Tariq Sadiq

Published: January 21, 2022
https://doi.org/10.1371/journal.pone.0262242
See the preprint

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Antimicrobial resistance
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Nanopore sequencing
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Polymerase chain reaction
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BLAST algorithm
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Ribosomal RNA
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Gene sequencing
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Sexually transmitted diseases
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Trichomonas vaginalis
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