An international, interlaboratory ring trial confirms the feasibility of an extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

• Margaret G. Mills ,

  Contributed equally to this work with: Margaret G. Mills, Emily Bruce

  Roles Formal analysis, Investigation, Methodology

  Affiliation Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America

  https://orcid.org/0000-0002-0493-9408

  ⨯
• Emily Bruce ,

  Contributed equally to this work with: Margaret G. Mills, Emily Bruce

  Roles Conceptualization, Formal analysis, Investigation, Writing – original draft, Writing – review & editing

  Affiliation Division of Immunobiology, Department of Medicine, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America

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• Meei-Li Huang,

  Roles Investigation, Methodology

  Affiliation Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America

  ⨯
• Jessica W. Crothers,

  Roles Investigation

  Affiliation Division of Immunobiology, Department of Medicine, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America

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• Ollivier Hyrien,

  Roles Data curation, Formal analysis, Methodology

  Affiliation Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

  ⨯
• Christopher A. L. Oura,

  Roles Investigation, Supervision

  Affiliation School of Veterinary Medicine, University of the West Indies, St. Augustine, Trinidad and Tobago

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• Lemar Blake,

  Roles Investigation

  Affiliation School of Veterinary Medicine, University of the West Indies, St. Augustine, Trinidad and Tobago

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• Arianne Brown Jordan,

  Roles Investigation

  Affiliation Ministry of Health, Port of Spain, Trinidad and Tobago

  https://orcid.org/0000-0002-4146-3920

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• Susan Hester,

  Roles Conceptualization, Investigation, Methodology, Writing – review & editing

  Affiliation Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, United States of America

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• Leah Wehmas,

  Roles Conceptualization, Investigation, Methodology, Writing – review & editing

  Affiliation Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, United States of America

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• Bernard Mari,

  Roles Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Université Côte d’Azur, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, FHU-OncoAge, Valbonne, France

  ⨯
• Pascal Barby,

  Roles Conceptualization, Investigation, Methodology, Writing – review & editing

  Affiliation Université Côte d’Azur, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, FHU-OncoAge, Valbonne, France

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• Caroline Lacoux,

  Roles Investigation

  Affiliation Université Côte d’Azur, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, FHU-OncoAge, Valbonne, France

  ⨯
• Julien Fassy,

  Roles Investigation

  Affiliation Université Côte d’Azur, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, FHU-OncoAge, Valbonne, France

  ⨯
• Pablo Vial,

  Roles Investigation, Methodology

  Affiliation Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, Chile

  https://orcid.org/0000-0002-4135-0416

  ⨯
• Cecilia Vial,

  Roles Investigation, Methodology

  Affiliation Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, Chile

  ⨯
• Jose R. W. Martinez,

  Roles Investigation

  Affiliation Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, Chile

  ⨯
• Olusola Olalekan Oladipo,

  Roles Data curation, Investigation, Methodology

  Affiliation Biochemistry Division, National Veterinary Research Institute, Vom, Nigeria

  ⨯
• Bitrus Inuwa,

  Roles Investigation, Methodology

  Affiliation Infectious and Transboundary Animal Diseases, National Veterinary Research Institute, Vom, Nigeria

  ⨯
• Ismaila Shittu,

  Roles Investigation, Methodology

  Affiliation Infectious and Transboundary Animal Diseases, National Veterinary Research Institute, Vom, Nigeria

  ⨯
• Clement A. Meseko,

  Roles Investigation, Methodology

  Affiliation Infectious and Transboundary Animal Diseases, National Veterinary Research Institute, Vom, Nigeria

  https://orcid.org/0000-0001-7003-7528

  ⨯
• Roger Chammas,

  Roles Data curation, Investigation, Methodology, Supervision, Writing – review & editing

  Affiliation Faculdade de Medicina da Universidade de São Paulo, Departamento de Radiologia e Oncologia, Centro de Investigação Translacional em Oncologia, Instituto do Cancer do Estado de São Paulo, São Paulo, Brazil

  https://orcid.org/0000-0003-0342-8726

  ⨯
• Carlos Ferreira Santos,

  Roles Investigation, Methodology

  Affiliation Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

  ⨯
• Thiago José Dionísio,

  Roles Investigation

  Affiliation Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

  ⨯
• Thais Francini Garbieri,

  Roles Investigation

  Affiliation Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

  ⨯
• Viviane Aparecida Parisi,

  Roles Investigation

  Affiliation Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil

  ⨯
• Maria Cassia Mendes-Correa,

  Roles Investigation

  Affiliation Department of Infectious Diseases, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil

  ⨯
• Anderson V. de Paula,

  Roles Investigation

  Affiliation Department of Infectious Diseases, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil

  ⨯
• Camila M. Romano,

  Roles Data curation, Investigation

  Affiliation Department of Infectious Diseases, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil

  ⨯
• Luiz Gustavo Bentim Góes,

  Roles Data curation, Investigation, Methodology

  Affiliations Scientific Platform Pasteur-USP, Universidade de São Paulo, São Paulo, Brazil, Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil

  ⨯
• Paola Minoprio,

  Roles Investigation

  Affiliation Scientific Platform Pasteur-USP, Universidade de São Paulo, São Paulo, Brazil

  ⨯
• Angelica C. Campos,

  Roles Investigation

  Affiliation Scientific Platform Pasteur-USP, Universidade de São Paulo, São Paulo, Brazil

  ⨯
• Marielton P. Cunha,

  Roles Investigation

  Affiliation Scientific Platform Pasteur-USP, Universidade de São Paulo, São Paulo, Brazil

  https://orcid.org/0000-0002-2102-631X

  ⨯
• Ana Paula P. Vilela,

  Roles Investigation

  Affiliation Scientific Platform Pasteur-USP, Universidade de São Paulo, São Paulo, Brazil

  ⨯
• Tonney Nyirenda,

  Roles Data curation, Investigation, Methodology

  Affiliation Department of Pathology, College of Medicine, University of Malawi, Blantyre, Malawi

  ⨯
• Rajhab Sawasawa Mkakosya,

  Roles Data curation, Investigation, Methodology

  Affiliation Department of Pathology, College of Medicine, University of Malawi, Blantyre, Malawi

  ⨯
• Adamson S. Muula,

  Roles Data curation, Investigation, Methodology, Project administration

  Affiliation Department of Public Health, College of Medicine, University of Malawi, Blantyre, Malawi

  ⨯
• Rebekah E. Dumm,

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation

  Affiliation Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

  ⨯
• Rebecca M. Harris,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology

  Affiliation Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

  ⨯
• Constance A. Mitchell,

  Roles Data curation, Project administration, Supervision, Writing – original draft, Writing – review & editing

  Affiliation Health and Environmental Sciences Institute, Washington, DC, United States of America

  ⨯
• Syril Pettit ,

  Roles Conceptualization, Data curation, Project administration, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: spettit@hesiglobal.org (SP); jason.botten@uvm.med.edu (JB); kjerome@uw.edu (KRJ)

  Affiliation Health and Environmental Sciences Institute, Washington, DC, United States of America

  https://orcid.org/0000-0002-4320-5343

  ⨯
• Jason Botten ,

  Roles Conceptualization, Data curation, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  * E-mail: spettit@hesiglobal.org (SP); jason.botten@uvm.med.edu (JB); kjerome@uw.edu (KRJ)

  Affiliations Division of Immunobiology, Department of Medicine, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America, Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America

  ⨯
•  [ ... ],
• Keith R. Jerome

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: spettit@hesiglobal.org (SP); jason.botten@uvm.med.edu (JB); kjerome@uw.edu (KRJ)

  Affiliations Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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