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Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
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Jun Yang,
Roles Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing
Affiliation Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China
⨯ - Nilakshi Barua,
- Md. Nannur Rahman,
- Norman Lo,
- Tsz Fung Tsang,
- Xiao Yang,
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Paul K. S. Chan,
Roles Conceptualization, Resources
Affiliation Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China
⨯ - Li Zhang,
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Margaret Ip
Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing
* E-mail: margaretip@cuhk.edu.hk
Affiliation Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong (SAR), China
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https://orcid.org/0000-0003-3739-7067
Chimeric crRNA improves CRISPR–Cas12a specificity in the N501Y mutation detection of Alpha, Beta, Gamma, and Mu variants of SARS-CoV-2
- Jun Yang,
- Nilakshi Barua,
- Md. Nannur Rahman,
- Norman Lo,
- Tsz Fung Tsang,
- Xiao Yang,
- Paul K. S. Chan,
- Li Zhang,
- Margaret Ip
- Published: December 23, 2021
- https://doi.org/10.1371/journal.pone.0261778