Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

Worarat Kruasuwan; Aekkachai Puseenam; Sutipa Tanapongpipat; Niran Roongsawang

doi:10.1371/journal.pone.0261754

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Open Access

Peer-reviewed

Research Article

Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

Worarat Kruasuwan,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
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Aekkachai Puseenam,

Roles Conceptualization, Data curation, Investigation, Methodology, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
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Sutipa Tanapongpipat,

Roles Conceptualization, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
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Niran Roongsawang

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft, Writing – review & editing

* E-mail: niran.roo@biotec.or.th

Affiliation Microbial Cell Factory Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand

https://orcid.org/0000-0002-2114-5052

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Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the thermotolerant methylotrophic yeast Ogataea thermomethanolica

Worarat Kruasuwan,
Aekkachai Puseenam,
Sutipa Tanapongpipat,
Niran Roongsawang

Published: December 23, 2021
https://doi.org/10.1371/journal.pone.0261754

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Guide RNA
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Yeast
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CRISPR
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Gene expression
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Secretion
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Nonsense mutation
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Saccharomyces cerevisiae
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