Primer extension coupled with fragment analysis for rapid and quantitative evaluation of 5.8S rRNA isoforms

Giulia Venturi; Federico Zacchini; Cinzia Lucia Vaccari; Davide Trerè; Lorenzo Montanaro

doi:10.1371/journal.pone.0261476

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Open Access

Peer-reviewed

Research Article

Primer extension coupled with fragment analysis for rapid and quantitative evaluation of 5.8S rRNA isoforms

Giulia Venturi,

Roles Conceptualization, Investigation, Validation, Writing – original draft

Affiliations Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum—Università di Bologna, Bologna, Italia, Centro di Ricerca Biomedica Applicata–CRBA, Università̀ di Bologna, Policlinico di Sant’Orsola, Bologna, Italia
⨯
Federico Zacchini,

Roles Investigation, Writing – review & editing

Affiliations Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum—Università di Bologna, Bologna, Italia, Centro di Ricerca Biomedica Applicata–CRBA, Università̀ di Bologna, Policlinico di Sant’Orsola, Bologna, Italia

https://orcid.org/0000-0002-6038-1779

⨯
Cinzia Lucia Vaccari,

Roles Investigation

Affiliations Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum—Università di Bologna, Bologna, Italia, Centro di Ricerca Biomedica Applicata–CRBA, Università̀ di Bologna, Policlinico di Sant’Orsola, Bologna, Italia
⨯
Davide Trerè,

Roles Formal analysis, Methodology, Writing – review & editing

Affiliations Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum—Università di Bologna, Bologna, Italia, Programma Dipartimentale di Medicina di Laboratorio, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italia
⨯
Lorenzo Montanaro

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

* E-mail: lorenzo.montanaro@unibo.it

Affiliations Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale (DIMES), Alma Mater Studiorum—Università di Bologna, Bologna, Italia, Centro di Ricerca Biomedica Applicata–CRBA, Università̀ di Bologna, Policlinico di Sant’Orsola, Bologna, Italia, Programma Dipartimentale di Medicina di Laboratorio, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italia

https://orcid.org/0000-0001-9776-9518

⨯

Primer extension coupled with fragment analysis for rapid and quantitative evaluation of 5.8S rRNA isoforms

Giulia Venturi,
Federico Zacchini,
Cinzia Lucia Vaccari,
Davide Trerè,
Lorenzo Montanaro

Published: December 21, 2021
https://doi.org/10.1371/journal.pone.0261476

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Ribosomal RNA
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Ribosomes
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Primer extension
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Nucleotides
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Nucleotide sequencing
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RNA analysis
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Reverse transcription
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