Selection and validation of reference genes for normalization of qRT-PCR data to study the cannabinoid pathway genes in industrial hemp

Michihito Deguchi; Shobha Potlakayala; Zachary Spuhler; Hannah George; Vijay Sheri; Ruba Agili; Aayushi Patel; Sairam Rudrabhatla

doi:10.1371/journal.pone.0260660

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Open Access

Peer-reviewed

Research Article

Selection and validation of reference genes for normalization of qRT-PCR data to study the cannabinoid pathway genes in industrial hemp

Michihito Deguchi,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Shobha Potlakayala,

Roles Data curation, Formal analysis, Methodology, Software, Writing – original draft, Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Zachary Spuhler,

Roles Investigation, Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Hannah George,

Roles Resources, Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Vijay Sheri,

Roles Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Ruba Agili,

Roles Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Aayushi Patel,

Roles Writing – review & editing

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America
⨯
Sairam Rudrabhatla

Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

* E-mail: svr11@psu.edu

Affiliation The Central Pennsylvania Research and Teaching Laboratory for Biofuels, Penn State Harrisburg, Middletown, Pennsylvania, United States of America

https://orcid.org/0000-0002-0663-7366

⨯

Selection and validation of reference genes for normalization of qRT-PCR data to study the cannabinoid pathway genes in industrial hemp

Michihito Deguchi,
Shobha Potlakayala,
Zachary Spuhler,
Hannah George,
Vijay Sheri,
Ruba Agili,
Aayushi Patel,
Sairam Rudrabhatla

Published: December 20, 2021
https://doi.org/10.1371/journal.pone.0260660

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