Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system

Worarat Kruasuwan; Aekkachai Puseenam; Chitwadee Phithakrotchanakoon; Sutipa Tanapongpipat; Niran Roongsawang

doi:10.1371/journal.pone.0258005

Loading metrics

Open Access

Peer-reviewed

Research Article

Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system

Worarat Kruasuwan,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Aekkachai Puseenam,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Chitwadee Phithakrotchanakoon,

Roles Data curation, Formal analysis, Investigation, Writing – review & editing

Affiliation Microbial Systems and Computational Biology Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Sutipa Tanapongpipat,

Roles Conceptualization, Writing – review & editing

Affiliation Microbial Cell Factory Research Team, Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand
⨯
Niran Roongsawang

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft, Writing – review & editing

* E-mail: niran.roo@biotec.or.th

Affiliation Microbial Cell Factory Research Team, Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand

https://orcid.org/0000-0002-2114-5052

⨯

Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system

Worarat Kruasuwan,
Aekkachai Puseenam,
Chitwadee Phithakrotchanakoon,
Sutipa Tanapongpipat,
Niran Roongsawang

Published: September 28, 2021
https://doi.org/10.1371/journal.pone.0258005

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Yeast
Is the Subject Area "Yeast" applicable to this article?

Thanks for your feedback.
Guide RNA
Is the Subject Area "Guide RNA" applicable to this article?

Thanks for your feedback.
Gene targeting
Is the Subject Area "Gene targeting" applicable to this article?

Thanks for your feedback.
Protein secretion
Is the Subject Area "Protein secretion" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.
Membrane proteins
Is the Subject Area "Membrane proteins" applicable to this article?

Thanks for your feedback.
Schizosaccharomyces pombe
Is the Subject Area "Schizosaccharomyces pombe" applicable to this article?

Thanks for your feedback.
Vacuoles
Is the Subject Area "Vacuoles" applicable to this article?

Thanks for your feedback.