Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk

• Sanoji Wijenayake ,

  Contributed equally to this work with: Sanoji Wijenayake, Shafinaz Eisha

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Center for Environmental Epigenetics and Development, Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada

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• Shafinaz Eisha ,

  Contributed equally to this work with: Sanoji Wijenayake, Shafinaz Eisha

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Center for Environmental Epigenetics and Development, Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada, Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada

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• Zoya Tawhidi,

  Roles Data curation

  Affiliation Center for Environmental Epigenetics and Development, Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada

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• Michael A. Pitino,

  Roles Methodology, Resources

  Affiliations Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada

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• Michael A. Steele,

  Roles Resources

  Affiliation Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada

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• Alison S. Fleming,

  Roles Funding acquisition

  Affiliation Department of Psychology, University of Toronto, Mississauga, Mississauga, Ontario, Canada

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• Patrick O. McGowan

  Roles Funding acquisition, Resources, Supervision, Writing – review & editing

  * E-mail: patrick.mcgowan@utoronto.ca

  Affiliations Center for Environmental Epigenetics and Development, Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada, Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada, Department of Psychology, University of Toronto, Toronto, Ontario, Canada, Department of Physiology, University of Toronto, Toronto, Ontario, Canada

  https://orcid.org/0000-0001-5459-5976

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