Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue

• Alireza Saraji ,

  Contributed equally to this work with: Alireza Saraji, Anne Offermann

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing – original draft

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

  https://orcid.org/0000-0003-3053-8373

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• Anne Offermann ,

  Contributed equally to this work with: Alireza Saraji, Anne Offermann

  Roles Conceptualization, Data curation, Investigation, Writing – review & editing

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Janine Stegmann-Frehse,

  Roles Conceptualization, Investigation, Methodology, Project administration

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Katharina Hempel,

  Roles Investigation, Methodology

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Duan Kang,

  Roles Data curation, Formal analysis, Methodology, Visualization, Writing – review & editing

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Rosemarie Krupar,

  Roles Data curation, Methodology, Writing – review & editing

  Affiliation Research Center Borstel, Leibniz Lung Center, Borstel, Germany

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• Christian Watermann,

  Roles Formal analysis, Methodology, Software, Writing – review & editing

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Danny Jonigk,

  Roles Data curation, Resources, Writing – review & editing

  Affiliations Institute of Pathology, Hannover Medical School, Hannover, Germany, Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), German Center for Lung Research, Hannover, Germany

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• Mark Philipp Kühnel,

  Roles Data curation, Methodology, Resources

  Affiliations Institute of Pathology, Hannover Medical School, Hannover, Germany, Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), German Center for Lung Research, Hannover, Germany

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• Jutta Kirfel,

  Roles Conceptualization, Validation, Writing – review & editing

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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• Sven Perner,

  Roles Conceptualization, Investigation, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliations Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany, Research Center Borstel, Leibniz Lung Center, Borstel, Germany

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• Verena Sailer

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: Verena-Wilbeth.Sailer@uksh.de

  Affiliation Pathology of the University Hospital Schleswig-Holstein, Campus Luebeck, Luebeck, Germany

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