Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista; Pedro M. Matos; Thomas J. A. Maguire; Kate Poulton; Elena Ortiz-Zapater; Robert Page; Helin Sertkaya; Ana M. Ortega-Prieto; Edward Scourfield; Aoife M. O’Byrne; Clement Bouton; Ruth E. Dickenson; Mattia Ficarelli; Jose M. Jimenez-Guardeño; Mark Howard; Gilberto Betancor; Rui Pedro Galao; Suzanne Pickering; Adrian W. Signell; Harry Wilson; Penelope Cliff; Mark Tan Kia Ik; Amita Patel; Eithne MacMahon; Emma Cunningham; Katie Doores; Monica Agromayor; Juan Martin-Serrano; Esperanza Perucha; Hannah E. Mischo; Manu Shankar-Hari; Rahul Batra; Jonathan Edgeworth; Mark Zuckerman; Michael H. Malim; Stuart Neil; Rocio Teresa Martinez-Nunez

doi:10.1371/journal.pone.0256813

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Open Access

Peer-reviewed

Research Article

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista ,

Contributed equally to this work with: Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton

Roles Data curation, Formal analysis, Methodology, Visualization, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Pedro M. Matos ,

Contributed equally to this work with: Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton

Roles Data curation, Formal analysis, Methodology, Visualization, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Thomas J. A. Maguire ,

Contributed equally to this work with: Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton

Roles Data curation, Formal analysis, Methodology, Visualization, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Inflammation Biology, School of Immunology and Microbial Sciences, Asthma UK Centre in Allergic Mechanisms of Asthma, King’s College London, London, United Kingdom

https://orcid.org/0000-0003-1034-4926

⨯
Kate Poulton ,

Contributed equally to this work with: Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton

Roles Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Elena Ortiz-Zapater,

Roles Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Randall Centre for Cell & Molecular Biophysics, King’s College London, London, United Kingdom, Peter Gorer Department of Immunobiology, King’s College London, London, United Kingdom

https://orcid.org/0000-0002-7437-4772

⨯
Robert Page,

Roles Data curation, Formal analysis, Methodology, Visualization, Writing – original draft

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, King’s Health Partners Integrated Cancer Centre, School of Cancer and Pharmaceutical Sciences, Guy’s Hospital, King’s College London, London, United Kingdom
⨯
Helin Sertkaya,

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Ana M. Ortega-Prieto,

Roles Formal analysis, Methodology, Visualization, Writing – review & editing

Affiliation Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0002-9023-0103

⨯
Edward Scourfield,

Roles Investigation

Affiliation Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Aoife M. O’Byrne,

Roles Data curation

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Centre for Inflammation Biology and Cancer Immunology (CIBCI), Centre for Rheumatic Diseases (CRD–EULAR Centre of Excellence), King’s College London, London, United Kingdom
⨯
Clement Bouton,

Roles Data curation

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Ruth E. Dickenson,

Roles Data curation, Methodology, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0003-0027-6317

⨯
Mattia Ficarelli,

Roles Data curation, Formal analysis, Methodology, Writing – original draft

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Jose M. Jimenez-Guardeño,

Roles Methodology, Writing – review & editing

Affiliation Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0002-1726-8033

⨯
Mark Howard,

Roles Methodology, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Peter Gorer Department of Immunobiology, King’s College London, London, United Kingdom

https://orcid.org/0000-0001-9779-5302

⨯
Gilberto Betancor,

Roles Methodology

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Rui Pedro Galao,

Roles Methodology, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0003-3368-5053

⨯
Suzanne Pickering,

Roles Methodology, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Adrian W. Signell,

Roles Methodology

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0003-4620-6891

⨯
Harry Wilson,

Roles Methodology

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0002-3185-1073

⨯
Penelope Cliff,

Roles Resources

Affiliation Viapath pathology laboratories at St Thomas’ Hospital, London, United Kingdom

https://orcid.org/0000-0001-6558-6039

⨯
Mark Tan Kia Ik,

Roles Methodology

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom

https://orcid.org/0000-0002-7774-3836

⨯
Amita Patel,

Roles Resources

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom

https://orcid.org/0000-0001-5945-7791

⨯
Eithne MacMahon,

Roles Resources

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom
⨯
Emma Cunningham,

Roles Resources

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom
⨯
Katie Doores,

Roles Funding acquisition, Methodology

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Monica Agromayor,

Roles Investigation, Methodology, Project administration, Supervision, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Juan Martin-Serrano,

Roles Resources

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Esperanza Perucha,

Roles Data curation, Investigation, Supervision, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Centre for Inflammation Biology and Cancer Immunology (CIBCI), Centre for Rheumatic Diseases (CRD–EULAR Centre of Excellence), King’s College London, London, United Kingdom

https://orcid.org/0000-0002-7802-0875

⨯
Hannah E. Mischo,

Roles Methodology, Supervision, Writing – original draft, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Manu Shankar-Hari,

Roles Funding acquisition

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Rahul Batra,

Roles Resources

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom
⨯
Jonathan Edgeworth,

Roles Funding acquisition, Resources

Affiliation Centre for Infectious Diseases Research, St Thomas’ Hospital, London, United Kingdom
⨯
Mark Zuckerman,

Roles Investigation, Resources, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, South London Specialist Virology Centre, King’s College Hospital, London, United Kingdom
⨯
Michael H. Malim,

Roles Conceptualization, Funding acquisition, Resources, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
Stuart Neil,

Roles Conceptualization, Funding acquisition, Resources, Writing – review & editing

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom
⨯
[ ... ],
Rocio Teresa Martinez-Nunez

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: rocio.martinez_nunez@kcl.ac.uk

Affiliations King’s College London Diagnostics Team at Guy’s Campus, London, United Kingdom, Dept. Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, London, United Kingdom

https://orcid.org/0000-0003-2507-4738

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Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista,
Pedro M. Matos,
Thomas J. A. Maguire,
Kate Poulton,
Elena Ortiz-Zapater,
Robert Page,
Helin Sertkaya,
Ana M. Ortega-Prieto,
Edward Scourfield,
Aoife M. O’Byrne

Published: September 15, 2021
https://doi.org/10.1371/journal.pone.0256813

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