Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes

Helena Rawsthorne-Manning; Fernando Calahorro; Patricia G. Izquierdo; Philippe Tardy; Thomas Boulin; Lindy Holden-Dye; Vincent O’Connor; James Dillon

doi:10.1371/journal.pone.0253351

Loading metrics

Open Access

Peer-reviewed

Research Article

Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes

Helena Rawsthorne-Manning,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0002-2284-8112

⨯
Fernando Calahorro,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0003-0659-7728

⨯
Patricia G. Izquierdo,

Roles Conceptualization, Methodology, Writing – review & editing

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0002-5491-0048

⨯
Philippe Tardy,

Roles Investigation, Methodology, Resources

Affiliation Institut NeuroMyoGène, Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Lyon, France
⨯
Thomas Boulin,

Roles Investigation, Methodology, Resources

Affiliation Institut NeuroMyoGène, Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U1217, Lyon, France
⨯
Lindy Holden-Dye,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0002-9704-1217

⨯
Vincent O’Connor,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0003-3185-5709

⨯
James Dillon

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: j.c.dillon@soton.ac.uk

Affiliation School of Biological Sciences, University of Southampton, Southampton, United Kingdom

https://orcid.org/0000-0003-3244-7483

⨯

Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes

Helena Rawsthorne-Manning,
Fernando Calahorro,
Patricia G. Izquierdo,
Philippe Tardy,
Thomas Boulin,
Lindy Holden-Dye,
Vincent O’Connor,
James Dillon

Published: January 18, 2022
https://doi.org/10.1371/journal.pone.0253351
See the preprint

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

CRISPRs
Is the Subject Area "CRISPRs" applicable to this article?

Thanks for your feedback.
Mutation
Is the Subject Area "Mutation" applicable to this article?

Thanks for your feedback.
Caenorhabditis elegans
Is the Subject Area "Caenorhabditis elegans" applicable to this article?

Thanks for your feedback.
Frameshift mutation
Is the Subject Area "Frameshift mutation" applicable to this article?

Thanks for your feedback.
Gene sequencing
Is the Subject Area "Gene sequencing" applicable to this article?

Thanks for your feedback.
DNA repair
Is the Subject Area "DNA repair" applicable to this article?

Thanks for your feedback.
Behavior
Is the Subject Area "Behavior" applicable to this article?

Thanks for your feedback.
Mutant strains
Is the Subject Area "Mutant strains" applicable to this article?

Thanks for your feedback.