Isolation of intact extracellular vesicles from cryopreserved samples

• Shannon N. Tessier,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Shriners Hospitals for Children—Boston, Boston, MA, United States of America

  https://orcid.org/0000-0003-2373-232X

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• Lauren D. Bookstaver,

  Roles Data curation, Formal analysis, Investigation, Methodology, Visualization

  Affiliation Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America

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• Cindy Angpraseuth,

  Roles Data curation, Investigation, Methodology

  Affiliation Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America

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• Cleo J. Stannard,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliation Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America

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• Beatriz Marques,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliation Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America

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• Uyen K. Ho,

  Roles Formal analysis, Investigation, Validation, Writing – review & editing

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Department of Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States of America

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• Alona Muzikansky,

  Roles Formal analysis, Methodology, Writing – review & editing

  Affiliation Biostatistics Center, Massachusetts General Hospital, Boston, MA, United States of America

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• Berent Aldikacti,

  Roles Data curation, Investigation, Methodology, Writing – original draft

  Current address: Department of Biochemistry and Molecular Biology, Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, MA, United States of America

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Department of Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States of America

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• Eduardo Reátegui,

  Roles Conceptualization, Methodology, Supervision

  Current address: William G. Lowrie Department of Chemical and Biomolecular Engineering, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States of America

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Department of Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States of America

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• Daniel C. Rabe,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – review & editing

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Department of Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States of America

  https://orcid.org/0000-0001-6126-2280

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• Mehmet Toner,

  Roles Conceptualization, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – review & editing

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Shriners Hospitals for Children—Boston, Boston, MA, United States of America

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• Shannon L. Stott

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: SSTOTT@mgh.harvard.edu

  Affiliations Department of Surgery, Center for Engineering in Medicine and BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America, Department of Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States of America

  https://orcid.org/0000-0002-0349-0522

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