Fit-for-purpose quantitative liquid biopsy based droplet digital PCR assay development for detection of programmed cell death ligand-1 (PD-L1) RNA expression in PAXgene blood samples

• Dennis O’Rourke ,

  Roles Conceptualization, Data curation, Formal analysis, Methodology, Resources, Validation, Writing – original draft, Writing – review & editing

  ¶‡ These authors share first authorship on this work and contributed equally to this work.

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

  https://orcid.org/0000-0002-9011-1032

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• Danyi Wang ,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  ¶‡ These authors share first authorship on this work and contributed equally to this work.

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

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• Jorge F. Sanchez-Garcia,

  Roles Data curation, Formal analysis, Methodology, Resources, Validation, Writing – original draft, Writing – review & editing

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

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• Maria Perella Cusano,

  Roles Methodology, Resources, Writing – original draft, Writing – review & editing

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

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• Waldemar Miller,

  Roles Data curation, Formal analysis, Visualization, Writing – review & editing

  Affiliation Biosample Informatics and Biobanking, Clinical Trial Management, Global Clinical Operations, Global Development, Merck KGaA, Darmstadt, Germany

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• Ti Cai,

  Roles Conceptualization, Funding acquisition, Methodology, Writing – review & editing

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

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• Juergen Scheuenpflug,

  Roles Conceptualization, Funding acquisition, Resources, Writing – review & editing

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, Merck KGaA, Darmstadt, Germany

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• Zheng Feng

  Roles Conceptualization, Data curation, Funding acquisition, Methodology, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: zheng.feng@emdserono.com

  Affiliation Global Clinical Biomarkers & Companion Diagnostics, Translational Medicine, Global Development, EMD Serono Research and Development Institute, Inc. a division of Merck KGaA, Billerica, MA, United States of America

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