RNAseq and RNA molecular barcoding reveal differential gene expression in cortical bone following hindlimb unloading in female mice

Jordan M. Spatz; Frank C. Ko; Ugur M. Ayturk; Matthew L. Warman; Mary L. Bouxsein

doi:10.1371/journal.pone.0250715

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Peer-reviewed

Research Article

RNAseq and RNA molecular barcoding reveal differential gene expression in cortical bone following hindlimb unloading in female mice

Jordan M. Spatz ,

Contributed equally to this work with: Jordan M. Spatz, Frank C. Ko

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliations Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America, University of California San Francisco School of Medicine, San Francisco, California, United States of America, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Frank C. Ko ,

Contributed equally to this work with: Jordan M. Spatz, Frank C. Ko

Roles Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

Affiliations Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America, Harvard Medical School, Boston, Massachusetts, United States of America

https://orcid.org/0000-0001-7305-7946

⨯
Ugur M. Ayturk,

Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

Affiliations Harvard Medical School, Boston, Massachusetts, United States of America, Boston Children’s Hospital, Boston, Massachusetts, United States of America
⨯
Matthew L. Warman,

Roles Formal analysis, Funding acquisition, Methodology, Supervision, Writing – review & editing

Affiliations Harvard Medical School, Boston, Massachusetts, United States of America, Boston Children’s Hospital, Boston, Massachusetts, United States of America
⨯
Mary L. Bouxsein

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing

* E-mail: mbouxsei@bidmc.harvard.edu

Affiliations Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America, Harvard Medical School, Boston, Massachusetts, United States of America
⨯

RNAseq and RNA molecular barcoding reveal differential gene expression in cortical bone following hindlimb unloading in female mice

Jordan M. Spatz,
Frank C. Ko,
Ugur M. Ayturk,
Matthew L. Warman,
Mary L. Bouxsein

Published: October 12, 2021
https://doi.org/10.1371/journal.pone.0250715

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RNA sequencing
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Gene expression
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Osteocytes
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Wnt signaling cascade
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Bone and joint mechanics
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Osteoblasts
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