Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli

Clément Madru; Ayten Dizkirici Tekpinar; Sandrine Rosario; Dariusz Czernecki; Sébastien Brûlé; Ludovic Sauguet; Marc Delarue

doi:10.1371/journal.pone.0250610

Loading metrics

Open Access

Peer-reviewed

Research Article

Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli

Clément Madru ,

Contributed equally to this work with: Clément Madru, Ayten Dizkirici Tekpinar

Roles Conceptualization, Investigation, Project administration, Writing – original draft, Writing – review & editing

Affiliation Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France

https://orcid.org/0000-0002-5412-8822

⨯
Ayten Dizkirici Tekpinar ,

Contributed equally to this work with: Clément Madru, Ayten Dizkirici Tekpinar

Roles Investigation, Writing – review & editing

Affiliations Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France, Department of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yıl University, Van, Turkey
⨯
Sandrine Rosario,

Roles Investigation

Affiliation Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France
⨯
Dariusz Czernecki,

Roles Investigation

Affiliations Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France, École Doctorale Complexité du Vivant, Sorbonne Université, Paris, France

https://orcid.org/0000-0002-3438-4722

⨯
Sébastien Brûlé,

Roles Investigation, Writing – review & editing

Affiliation Molecular Biophysics Platform, C2RT, Institut Pasteur, CNRS UMR, Paris, France

https://orcid.org/0000-0002-8797-6748

⨯
Ludovic Sauguet ,

Roles Conceptualization, Funding acquisition, Methodology, Validation, Writing – review & editing

* E-mail: ludovic.sauguet@pasteur.fr (LS); marc.delarue@pasteur.fr (MD)

Affiliation Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France
⨯
Marc Delarue

Roles Conceptualization, Funding acquisition, Supervision, Validation, Writing – review & editing

* E-mail: ludovic.sauguet@pasteur.fr (LS); marc.delarue@pasteur.fr (MD)

Affiliation Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France
⨯

Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli

Clément Madru,
Ayten Dizkirici Tekpinar,
Sandrine Rosario,
Dariusz Czernecki,
Sébastien Brûlé,
Ludovic Sauguet,
Marc Delarue

Published: April 29, 2021
https://doi.org/10.1371/journal.pone.0250610

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

SARS CoV 2
Is the Subject Area "SARS CoV 2" applicable to this article?

Thanks for your feedback.
Escherichia coli
Is the Subject Area "Escherichia coli" applicable to this article?

Thanks for your feedback.
COVID 19
Is the Subject Area "COVID 19" applicable to this article?

Thanks for your feedback.
Primer extension assay
Is the Subject Area "Primer extension assay" applicable to this article?

Thanks for your feedback.
Sedimentation
Is the Subject Area "Sedimentation" applicable to this article?

Thanks for your feedback.
Stoichiometry
Is the Subject Area "Stoichiometry" applicable to this article?

Thanks for your feedback.
Imidazole
Is the Subject Area "Imidazole" applicable to this article?

Thanks for your feedback.
Recombinant proteins
Is the Subject Area "Recombinant proteins" applicable to this article?

Thanks for your feedback.