High-throughput screening of the ReFRAME, Pandemic Box, and COVID Box drug repurposing libraries against SARS-CoV-2 nsp15 endoribonuclease to identify small-molecule inhibitors of viral activity

Ryan Choi; Mowei Zhou; Roger Shek; Jesse W. Wilson; Logan Tillery; Justin K. Craig; Indraneel A. Salukhe; Sarah E. Hickson; Neeraj Kumar; Rhema M. James; Garry W. Buchko; Ruilian Wu; Sydney Huff; Tu-Trinh Nguyen; Brett L. Hurst; Sara Cherry; Lynn K. Barrett; Jennifer L. Hyde; Wesley C. Van Voorhis

doi:10.1371/journal.pone.0250019

Loading metrics

Open Access

Peer-reviewed

Research Article

High-throughput screening of the ReFRAME, Pandemic Box, and COVID Box drug repurposing libraries against SARS-CoV-2 nsp15 endoribonuclease to identify small-molecule inhibitors of viral activity

Ryan Choi,

Roles Conceptualization, Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America
⨯
Mowei Zhou,

Roles Formal analysis, Investigation, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory (PNNL), Richland, WA, United States of America
⨯
Roger Shek,

Roles Investigation, Writing – review & editing

Affiliations Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America
⨯
Jesse W. Wilson,

Roles Investigation, Validation

Affiliation Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory (PNNL), Richland, WA, United States of America
⨯
Logan Tillery,

Roles Investigation

Affiliations Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America

https://orcid.org/0000-0002-5431-3489

⨯
Justin K. Craig,

Roles Investigation, Writing – review & editing

Affiliations Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America
⨯
Indraneel A. Salukhe,

Roles Conceptualization, Investigation, Visualization, Writing – review & editing

Affiliation Department of Microbiology, University of Washington School of Medicine, Seattle, WA, United States of America
⨯
Sarah E. Hickson,

Roles Investigation, Writing – review & editing

Affiliation Department of Microbiology, University of Washington School of Medicine, Seattle, WA, United States of America
⨯
Neeraj Kumar,

Roles Investigation, Visualization, Writing – review & editing

Affiliation Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory (PNNL), Richland, WA, United States of America
⨯
Rhema M. James,

Roles Investigation

Affiliation Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory (PNNL), Richland, WA, United States of America
⨯
Garry W. Buchko,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliations Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory (PNNL), Richland, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America, School of Molecular Bioscience, Washington State University, Pullman, WA, United States of America

https://orcid.org/0000-0002-3639-1061

⨯
Ruilian Wu,

Roles Investigation, Resources

Affiliation Bioenergy and Biome Sciences, Los Alamos National Laboratory (LANL), Los Alamos, NM, United States of America
⨯
Sydney Huff,

Roles Investigation, Writing – review & editing

Affiliation Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America
⨯
Tu-Trinh Nguyen,

Roles Resources

Affiliation Calibr, a division of The Scripps Research Institute, La Jolla, CA, United States of America
⨯
Brett L. Hurst,

Roles Formal analysis, Investigation

Affiliation Institute for Antiviral Research, Utah State University, Logan, UT, United States of America
⨯
Sara Cherry,

Roles Formal analysis, Investigation, Visualization, Writing – original draft

Affiliation Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, United States of America
⨯
Lynn K. Barrett,

Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

Affiliations Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America
⨯
Jennifer L. Hyde,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliation Department of Microbiology, University of Washington School of Medicine, Seattle, WA, United States of America
⨯
[ ... ],
Wesley C. Van Voorhis

Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review & editing

* E-mail: wesley@uw.edu

Affiliations Division of Allergy and Infectious Diseases, Department of Medicine, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington School of Medicine, Seattle, WA, United States of America, Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA, United States of America, Department of Microbiology, University of Washington School of Medicine, Seattle, WA, United States of America, Department of Global Health, University of Washington, Seattle, WA, United States of America

https://orcid.org/0000-0001-6141-2015

⨯
[ view all ]
[ view less ]

High-throughput screening of the ReFRAME, Pandemic Box, and COVID Box drug repurposing libraries against SARS-CoV-2 nsp15 endoribonuclease to identify small-molecule inhibitors of viral activity

Ryan Choi,
Mowei Zhou,
Roger Shek,
Jesse W. Wilson,
Logan Tillery,
Justin K. Craig,
Indraneel A. Salukhe,
Sarah E. Hickson,
Neeraj Kumar,
Rhema M. James

Published: April 22, 2021
https://doi.org/10.1371/journal.pone.0250019

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

SARS CoV 2
Is the Subject Area "SARS CoV 2" applicable to this article?

Thanks for your feedback.
Fluorescence resonance energy transfer
Is the Subject Area "Fluorescence resonance energy transfer" applicable to this article?

Thanks for your feedback.
Monomers
Is the Subject Area "Monomers" applicable to this article?

Thanks for your feedback.
Pandemics
Is the Subject Area "Pandemics" applicable to this article?

Thanks for your feedback.
Coronaviruses
Is the Subject Area "Coronaviruses" applicable to this article?

Thanks for your feedback.
Crystal structure
Is the Subject Area "Crystal structure" applicable to this article?

Thanks for your feedback.
Library screening
Is the Subject Area "Library screening" applicable to this article?

Thanks for your feedback.
RNA structure
Is the Subject Area "RNA structure" applicable to this article?

Thanks for your feedback.