Development of a loop-mediated isothermal amplification assay combined with a nanoparticle-based lateral flow biosensor for rapid detection of plasmid-mediated colistin resistance gene mcr-1

Lin Gong; Fei Tang; Ernan Liu; Xiaoli Liu; Huiqiong Xu; Yimei Wang; Yadong Song; Jiansheng Liang

doi:10.1371/journal.pone.0249582

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Open Access

Peer-reviewed

Research Article

Development of a loop-mediated isothermal amplification assay combined with a nanoparticle-based lateral flow biosensor for rapid detection of plasmid-mediated colistin resistance gene mcr-1

Lin Gong ,

Contributed equally to this work with: Lin Gong, Fei Tang, Ernan Liu

Roles Data curation, Methodology, Writing – original draft

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Fei Tang ,

Contributed equally to this work with: Lin Gong, Fei Tang, Ernan Liu

Roles Conceptualization, Writing – review & editing

Affiliation MOE Key Laboratory of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China
⨯
Ernan Liu ,

Contributed equally to this work with: Lin Gong, Fei Tang, Ernan Liu

Roles Formal analysis, Investigation, Methodology, Project administration

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Xiaoli Liu,

Roles Conceptualization, Project administration

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Huiqiong Xu,

Roles Investigation, Methodology

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Yimei Wang,

Roles Investigation, Methodology

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Yadong Song,

Roles Investigation

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China
⨯
Jiansheng Liang

Roles Conceptualization, Project administration, Supervision, Writing – review & editing

* E-mail: wh_ljs@sina.com

Affiliation Department of Disinfection and Pest Control, Wuhan Center for Disease Control and Prevention, Wuhan, People’s Republic of China

https://orcid.org/0000-0001-5001-2825

⨯

Development of a loop-mediated isothermal amplification assay combined with a nanoparticle-based lateral flow biosensor for rapid detection of plasmid-mediated colistin resistance gene mcr-1

Lin Gong,
Fei Tang,
Ernan Liu,
Xiaoli Liu,
Huiqiong Xu,
Yimei Wang,
Yadong Song,
Jiansheng Liang

Published: April 15, 2021
https://doi.org/10.1371/journal.pone.0249582

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