Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization

• Clyde S. Manuel ,

  Roles Conceptualization, Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing

  * E-mail: mdmoore@umass.edu (MDM); ManuelC@gojo.com (CSM)

  Current address: GOJO Industries, Akron, OH, United States of America

  Affiliation Department of Food, Nutrition, and Bioprocessing Sciences, North Carolina State University, Raleigh, NC, United States of America

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• Cassandra Suther ,

  Contributed equally to this work with: Cassandra Suther, Matthew D. Moore, Lee-Ann Jaykus

  Roles Methodology, Writing – original draft, Writing – review & editing

  Affiliation Department of Food Science, University of Massachusetts, Amherst, MA, United States of America

  https://orcid.org/0000-0002-8861-6883

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• Matthew D. Moore ,

  Contributed equally to this work with: Cassandra Suther, Matthew D. Moore, Lee-Ann Jaykus

  Roles Writing – original draft, Writing – review & editing

  * E-mail: mdmoore@umass.edu (MDM); ManuelC@gojo.com (CSM)

  Affiliations Department of Food, Nutrition, and Bioprocessing Sciences, North Carolina State University, Raleigh, NC, United States of America, Department of Food Science, University of Massachusetts, Amherst, MA, United States of America

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• Lee-Ann Jaykus

  Contributed equally to this work with: Cassandra Suther, Matthew D. Moore, Lee-Ann Jaykus

  Roles Conceptualization, Funding acquisition, Investigation, Project administration, Writing – review & editing

  Affiliation Department of Food, Nutrition, and Bioprocessing Sciences, North Carolina State University, Raleigh, NC, United States of America

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