Establishment of a novel probe-based RT-qPCR approach for detection and quantification of tight junctions reveals age-related changes in the gut barriers of broiler chickens

• J. Sophia von Buchholz,

  Roles Investigation, Methodology, Writing – original draft

  Affiliation Department for Farm Animals and Veterinary Public Health, Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria

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• Ivana Bilic,

  Roles Methodology

  Affiliation Department for Farm Animals and Veterinary Public Health, Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria

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• Jörg R. Aschenbach,

  Roles Supervision, Visualization

  Affiliation Department of Veterinary Medicine, Institute of Veterinary Physiology, Freie Universität Berlin, Berlin, Germany

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• Michael Hess,

  Roles Supervision, Validation, Visualization, Writing – review & editing

  Affiliation Department for Farm Animals and Veterinary Public Health, Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria

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• Taniya Mitra,

  Roles Methodology

  Affiliation Department for Farm Animals and Veterinary Public Health, Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria

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• Wageha A. Awad

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Validation, Visualization, Writing – review & editing

  * E-mail: wageha.awad@vetmeduni.ac.at

  Affiliation Department for Farm Animals and Veterinary Public Health, Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria

  https://orcid.org/0000-0003-0843-2089

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