DNA vaccine candidate encoding SARS-CoV-2 spike proteins elicited potent humoral and Th1 cell-mediated immune responses in mice

Eakachai Prompetchara; Chutitorn Ketloy; Kittipan Tharakhet; Papatsara Kaewpang; Supranee Buranapraditkun; Teerasit Techawiwattanaboon; Suwitra Sathean-anan-kun; Patrawadee Pitakpolrat; Supaporn Watcharaplueksadee; Supaporn Phumiamorn; Wassana Wijagkanalan; Kanitha Patarakul; Tanapat Palaga; Kiat Ruxrungtham

doi:10.1371/journal.pone.0248007

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Open Access

Peer-reviewed

Research Article

DNA vaccine candidate encoding SARS-CoV-2 spike proteins elicited potent humoral and Th1 cell-mediated immune responses in mice

Eakachai Prompetchara,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Integrated Frontier Biotechnology for Emerging Disease, Chulalongkorn University, Bangkok, Thailand

https://orcid.org/0000-0001-7565-9211

⨯
Chutitorn Ketloy ,

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Writing – review & editing

* E-mail: Chutitorn.K@chula.ac.th

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Integrated Frontier Biotechnology for Emerging Disease, Chulalongkorn University, Bangkok, Thailand

https://orcid.org/0000-0002-7043-8062

⨯
Kittipan Tharakhet,

Roles Data curation, Investigation, Methodology, Visualization

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Papatsara Kaewpang,

Roles Data curation, Investigation, Methodology

Affiliation Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Supranee Buranapraditkun,

Roles Data curation, Formal analysis, Investigation, Methodology

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Teerasit Techawiwattanaboon,

Roles Data curation, Formal analysis, Investigation, Methodology

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

https://orcid.org/0000-0002-8188-9934

⨯
Suwitra Sathean-anan-kun,

Roles Investigation, Methodology

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Patrawadee Pitakpolrat,

Roles Investigation, Methodology

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Supaporn Watcharaplueksadee,

Roles Investigation, Methodology

Affiliation Thai Red Cross Emerging Infectious Diseases-Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Supaporn Phumiamorn,

Roles Investigation, Methodology

Affiliation Institute of Biological Product, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand
⨯
Wassana Wijagkanalan,

Roles Conceptualization, Resources

Affiliation BioNet-Asia Co., Ltd, Bangkok, Thailand
⨯
Kanitha Patarakul,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Integrated Frontier Biotechnology for Emerging Disease, Chulalongkorn University, Bangkok, Thailand, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
⨯
Tanapat Palaga,

Roles Conceptualization, Supervision, Writing – review & editing

Affiliations Integrated Frontier Biotechnology for Emerging Disease, Chulalongkorn University, Bangkok, Thailand, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
⨯
Kiat Ruxrungtham

Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

Affiliations Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, Integrated Frontier Biotechnology for Emerging Disease, Chulalongkorn University, Bangkok, Thailand
⨯

DNA vaccine candidate encoding SARS-CoV-2 spike proteins elicited potent humoral and Th1 cell-mediated immune responses in mice

Eakachai Prompetchara,
Chutitorn Ketloy,
Kittipan Tharakhet,
Papatsara Kaewpang,
Supranee Buranapraditkun,
Teerasit Techawiwattanaboon,
Suwitra Sathean-anan-kun,
Patrawadee Pitakpolrat,
Supaporn Watcharaplueksadee,
Supaporn Phumiamorn

Published: March 22, 2021
https://doi.org/10.1371/journal.pone.0248007

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