Flow Assisted Mutation Enrichment (FAME): A highly efficacious and efficient method to enrich Double Knockouts (DKO) after gene editing

• Michael Hansen,

  Roles Data curation, Investigation, Methodology, Project administration

  Affiliation Division of Endocrinology, Phoenix VA Medical Center, Phoenix, Arizona, United States of America

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• Xiaopin Cai,

  Roles Methodology, Project administration

  Current address: Division of Endocrinology, China-Japan Friendship Hospital, Beijing, China

  Affiliation Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America

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• Sara Bowen,

  Roles Formal analysis, Methodology, Project administration, Software, Validation, Visualization

  Affiliation Flow Cytometry Core Facility, Barrow Neurological Institute, Phoenix, Arizona, United States of America

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• David A. Largaespada,

  Roles Funding acquisition, Resources, Supervision, Writing – review & editing

  Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, United States of America

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• Ming V. Li

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: Ming.Li@va.gov

  Affiliations Division of Endocrinology, Phoenix VA Medical Center, Phoenix, Arizona, United States of America, Department of Medicine, University of Arizona College of Medicine, Phoenix, Arizona, United States of America

  https://orcid.org/0000-0003-3740-5354

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