An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes

• Cristina Leoni ,

  Contributed equally to this work with: Cristina Leoni, Niccolò Bianchi

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: cristina.leoni@irb.usi.ch (CL); silvia.monticelli@irb.usi.ch (SM)

  Affiliation Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland

  https://orcid.org/0000-0002-4458-1121

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• Niccolò Bianchi ,

  Contributed equally to this work with: Cristina Leoni, Niccolò Bianchi

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland, Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland

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• Lucia Vincenzetti,

  Roles Investigation, Methodology

  Affiliation Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland

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• Silvia Monticelli

  Roles Conceptualization, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: cristina.leoni@irb.usi.ch (CL); silvia.monticelli@irb.usi.ch (SM)

  Affiliation Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland

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